apoptic death agonist | BH3 interacting domain death agonist | BH3-interacting domain death agonist | BID isoform ES(1b) | BID isoform L(2) | BID isoform Si6 | BID | desmocollin type 4 | FP497 | Human BID coding sequence | MGC15319 | MGC42355 | p22 BID |
规格或纯度
ActiBioPure™, 生物活性, 无载体, ≥95%(SDS-PAGE)
生化机理
BID is a 195 amino acid member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. BID is a pro‑apoptotic member that causes cytochrome c to be released from the mitochondria intermembrane space into the cytosol. In healthy cells BID is cytosolic. In response to Fas ligand or TNF, BID is cleaved by caspase-8 and it then relocates to the mitochondria outer membrane. Cleavage of BID by caspase-8 generates a new N-terminus that contains a terminal glycine. It appears that the glycine is myristoylated and myristoylation serves to target BID to the mitochondria. BID may then interact with another pro‑apoptotic Bcl-2 family member Bak. Interaction of BID with Bak causes altered mitochondrial membrane permeability. A 9-13 amino acid stretch called the BH3 region (Bcl-2 homology region) appears to mediate the BID interaction with other Bcl-2 family members. BID is neutralized by binding to the anti-apoptotic member Bcl‑xL.
Post-translational: BH3-interacting domain death agonist: TNF-alpha induces caspase-mediated cleavage into a major p15 and minor p13 and p11 products (By similarity). Cleaved by CASP6 into a major p15 and minor p13 products, leading to release of cytochrome c and subsequent nonalcoholic steatohepatitis (PubMed:32029622). BH3-interacting domain death agonist p15: Ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.
生物活性
Immobilized Recombinant Human BID Protein (rp183602) at 2.0 μg/mL can bind Recombinant Human Bcl-XL Protein (rp209934) with the EC50 of 261.4 ng/mL.
19.3 kDa, under reducing conditions; 19.3 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 20mM Tris, 500mM NaCl, pH 8.0.
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human BID Protein (rp183602) - Binding Activity Immobilized Recombinant Human BID Protein (rp183602) at 2.0 μg/mL can bind Recombinant Human Bcl-XL Protein (rp209934) with the EC
₅₀
of 261.4 ng/mL.
Recombinant Human BID Protein (rp183602) - SDS-PAGE 3 μg/lane of Recombinant Human BID Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 19.3 kDa.
ZJ24F1113646
危险品化学品经营许可证（带存储）