The macrophage inflammatory protein-1 alpha (MIP-1 alpha )/CCL3 and-1 beta (MIP-1 beta )/CCL4 are highly homologous CC family chemokines that were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Mouse MIP-1 alpha and MIP-1 beta are encoded by their respective single-copy genes. In human, additional non-allelic MIP-1 alpha and MIP-1 beta genes also exist. The two human MIP-1 alpha genes arise by duplication/mutation. They code for MIP-1 alpha isoforms CCL3/LD78 alpha and CCL3L1/LD78 beta, which share 94% amino acid (aa) sequence homology. Whereas the human CCL3/LD78 alpha is a single-copy gene, the human CCL3L1/LD78 beta gene copy number varies within the population. Human CCL3L1/LD78 beta cDNA encodes a 93 aa residue precursor with a 23 aa residue signal peptide that is cleaved to generate a 70 aa mature protein. Human CCL3L1/LD78 beta binds and signals through chemokine receptors CCR1 and CCR5. When compared to CCL3/LD78 alpha, CCL3L1/LD78 beta has higher binding affinity to CCR5, which also functions as a coreceptor for HIV-1 entry. The copy number of CCL3L1 is one of several genetic determinants of HIV-1 susceptibility.
Post-translational: The N-terminal processed forms LD78-beta(3-70) and LD78-beta(5-70) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. The cleavage to yield LD78-beta(3-70) is probably achieved by DPP4.
生物活性
Determined by its ability to chemoattract human monocytes using a concentration range of 0 - 5.0 ng/mL.
10.0 & 16.8 kDa, under reducing conditions; 10.8 & 19.0 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human CCL3L1/LD78 beta Protein (rp183664) - SDS-PAGE Determined by its ability to chemoattract human monocytes using a concentration range of 0 - 5.0 ng/mL.
Recombinant Human CCL3L1/LD78 beta Protein (rp183664) - SDS-PAGE 3 μg/lane of Recombinant Human CCL3L1/LD78 beta Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 10.0 & 16.8 kDa under reducing conditions and 10.8 & 19.0 kDa under non-reducing conditions. CCL3L1/LD78 beta protein is a member of the CC chemokine family. These proteins often form multimers through disulfide bonds (Uniprot: P16619).