无载体, 生物活性, ActiBioPure™, 高性能, ≥95%(SDS-PAGE), See COA
生化机理
IFN gamma, also known as IFNG, is a secreted protein that belongs to the type II interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and tumor control. Aberrant IFN gamma expression is associated with some autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.
Post-translational: Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
生物活性
Measured by its ability to inhibit proliferation of L‑929 mouse fibroblast cells. The ED₅₀ for this effect is 8.5 pg/mL.
12.5 kDa, under reducing conditions; 12.7 & 26.8 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, 10% Trehalose, 5% Mannitol, pH 7.4.
浓度
See COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Rat IFN-gamma Protein (rp186511) - Protein Bioactivity
Measured by its ability to inhibit proliferation of L‑929 mouse fibroblast cells. The ED₅₀ for this effect is 8.5 pg/mL.
Recombinant Rat IFN-gamma Protein (rp186511) - SDS-PAGE 3 μg/lane of Recombinant Rat IFN-gamma Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 12.5 kDa under reducing conditions and 12.7 & 26.8 kDa under non-reducing conditions. IFNγ has a characteristic domain-swapped dimer structure with two of the six α-helices exchanged with each other. (PMID: 38879015)