Recombinant Rat IFN-gamma Protein

货号: rp186511
有货
级别和纯度: 无载体 ? 无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。 生物活性 ? 生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。 ActiBioPure™ ? ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。 高性能 ? 高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。 ≥95%(SDS-PAGE) See COA
Accession #
P01581
蛋白标签
No tag
表达系统
E. coli
内毒素水平
<1.0 EU/μg
生物活性
Measured by its ability to inhibit proliferation of L‑929 mouse fibroblast cells. The ED₅₀ for this effect is 8.5 pg/mL.
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规格
库存
价格
数量
10μg
rp186511-10μg
现货 Stock Image
¥339.90
50μg
rp186511-50μg
现货 Stock Image
¥999.90
100μg
rp186511-100μg
现货 Stock Image
¥1,629.90
1mg
rp186511-1mg
期货 Stock Image
¥10,699.90
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为什么选择此级别

ActiBioPure™,无载体,生物活性,高性能 级 提供 ≥95%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

规格

产品名称
Recombinant Rat IFN-gamma Protein
别名
重组大鼠IFN-gamma蛋白 | 重组大鼠干扰素 γ 蛋白
英文别名
IFG | IFI | IFNG | IFNgamma | IFN-gamma | Immune interferon | interferon gamma | interferon, gamma
规格或纯度
无载体, 生物活性, ActiBioPure™, 高性能, ≥95%(SDS-PAGE), See COA
生化机理
IFN gamma, also known as IFNG, is a secreted protein that belongs to the type II interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and tumor control. Aberrant IFN gamma expression is associated with some autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity. Post-translational: Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
生物活性
Measured by its ability to inhibit proliferation of L‑929 mouse fibroblast cells. The ED₅₀ for this effect is 8.5 pg/mL.
表达系统
E. coli
种属
大鼠(Rat)
氨基酸
23-156 aa
序列
QGTLIESLESLKNYFNSSSMDAMEGKSLLLDIWRNWQKDGNTKILESQIISFYLRLFEVLKDNQAISNNISVIESHLITNFFSNSKAKKDAFMSIAKFEVNNPQIQHKAVNELIRVIHQLSPESSLRKRKRSRC​
蛋白标签
No tag
无载体
Yes
无动物源
No
来源
重组表达
预测分子量
29.2 kDa
SDS-PAGE
12.5 kDa, under reducing conditions; 12.7 & 26.8 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, 10% Trehalose, 5% Mannitol, pH 7.4.
浓度
See COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片

Recombinant Rat IFN-gamma Protein (rp186511) - Protein Bioactivity

Measured by its ability to inhibit proliferation of L‑929 mouse fibroblast cells. The ED₅₀ for this effect is 8.5 pg/mL.

Recombinant Rat IFN-gamma Protein (rp186511) - SDS-PAGE
3 μg/lane of Recombinant Rat IFN-gamma Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 12.5 kDa under reducing conditions and 12.7 & 26.8 kDa under non-reducing conditions.
IFNγ has a characteristic domain-swapped dimer structure with two of the six α-helices exchanged with each other. (PMID: 38879015)

技术文档

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高级数据

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批号(Lot Number) 证书类型 货号
ZJ25F0420953 分析证书 rp186511
ZJ25F0420954 分析证书 rp186511
ZJ25F0420955 分析证书 rp186511
技术文档和文章
溶液计算器