CDABP0042 | CDC 47 | CDC47 | CDC47 homolog | Cdc47, S. cerevisiae, homolog of | DNA replication licensing factor MCM7 | Homolog of S. cerevisiae Cdc47 | MCM 2 | MCM 7 | MCM2 | MCM2, formerly | Mcm7 | MCM7 minichromosome maintenance deficient 7 | MCM7_HUMA
规格或纯度
无载体,His标签,≥90%(SDS-PAGE),见COA
生化机理
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
Post-translational: Phosphorylated upon DNA damage, probably by ATM or ATR.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human MCM7 Protein (rp217597) - SDS-PAGE 3 μg/lane of Recombinant Human MCM7 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 17.0 & 34.9 kDa. MCM7 Protein yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance (Uniprot: P33993).
ZJ25F0724465
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