Interferon, alpha 5 (IFNA5) belongs to the alpha/beta interferon family. IFNA5 is the only IFNA subtype detected in normal liver, while a mixture of subtypes is observed in the liver tissue of patients with chronic hepatitis C. Interferons are produced by macrophages, IFN-alpha has antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha, the first cytokine to be produced by recombinant DNA technology, has emerged as an important regulator of growth and differentiation, affecting cellular communication and signal transduction pathways as well as immunological control. Originally discovered as an antiviral substance, the efficacy of IFN-alpha in malignant, viral, immunological, angiogenic, inflammatory, and fibrotic diseases suggests a spectrum of interrelated pathophysiologies. IFN-alpha emerged as a prototypic tumor suppressor protein that represses the clinical tumorigenic phenotype in some malignancies capable of differentiation.
生物活性
Recombinant human IFN-α bioactivity is measured by inhibition of the proliferation of HT-29 cells.The EC50 for this effect is typically 0.07 ng/mL.
48.1 kDa, under reducing conditions; 45. 0 & 79.0 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4.
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human IFN-alpha G/IFNA5 Protein (rp222555) - SDS-PAGE
3 μg/lane of Recombinant Human IFN-alpha G/IFNA5 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 48.1 kDa under reducing conditions and 79.0 kDa under non-reducing conditions.
There are multiple pairs of disulfide bonds in the protein sequence. Disulfide bonds cause the protein to present as a polymer in a non-reducing conditions (Uniprot ID: P01569).
Recombinant Human IFN-alpha G/IFNA5 Protein (rp222555) - Protein Bioactivity
Recombinant human IFN-alpha G/IFNA5 protein bioactivity is measured by inhibition of the proliferation of HT-29 cells. The EC50 for this effect is typically 0.07 ng/mL.
ZJ26F0231946
危险品化学品经营许可证（带存储）