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LNP 干细胞RNA转染试剂盒
有货
为什么选择此级别
BioReagent,分子生物学级,无菌 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,禁止冷冻。低温运输,禁止冷冻 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
LNP干细胞 RNA 转染试剂盒是一种基于脂质纳米粒（LNP）技术研发的新型即用型转染试剂，利用 LNP 独特的跨膜转运和内体逃逸机制，实现对 RNA 高效递送，广泛适用于不同类型 RNA 在 MSC 等干细胞中的转染。
易用：无需特定包封设备，一步混合即可实现 RNA-LNP 瞬时包封，无需孵育，可立即进行转染，转染后无需更换培养基。
高效：微量样品的包封率可达 80%以上，实现对 MSC 等干细胞的高效转染。
安全：本品不含乙醇，采用新型生物可降解脂质材料，细胞毒性极低，且经 0.2μm 微孔滤膜过滤。
产品组分表
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运输与保存方法
受控常温(≤25℃)运输。2℃~8℃保存。不得冷冻！
适用范围
LNP干细胞 RNA 转染试剂盒适用于 MSC 等干细胞 RNA 体外转染，其中 RNA 类型包括 siRNA、miRNA、gRNA、mRNA、saRNA 和 circRNA 等。
本产品仅供研究使用。
注意事项
（1）RNA 原液的最佳溶剂为无酶水，且 mRNA 浓度不低于 200ng/μL、短链 RNA 浓度不低于 100ng/μL、siRNA 浓度不低于 10μM，其他类型的溶剂对转染效果可能会有一定影响，具体情况可以联系我们。
（2）制备 RNA 预混液请确保使用试剂盒专用 Buffer 稀释 RNA 原液，不得使用 PBS 或培养基。
（3）请确保 RNA 预混液浓度为 40ng/μL。若多种 RNA 共转染，如 CRISPR Cas9 mRNA 与 gRNA 共转染，则 RNA 预混液浓度为 Cas9 mRNA + gRNA 的总浓度，Cas9 mRNA 与 gRNA 的推荐用量比为 1:1～1:2（μgmRNA :μggRNA）。
（4）RNA/LNP 的比例是 LNP 包封的关键因素，如需增减每孔 RNA 用量，请按以下操作进行：
1、对于 mRNA 转染和 mRNA+gRNA 共转染，如需增减每孔 mRNA 用量，请按比例增加或减少加入细胞的每孔 RNA-LNP复合物的体积。
2、对于 siRNA 转染，如需增加每孔 siRNA 用量，请按比例增加加入细胞的每孔 RNA-LNP复合物的体积。 如需减少每孔 siRNA 用量，LNP转染试剂的最低用量应不低于推荐用量 II。以 96 孔为例，当 siRNA 每孔用量降为 0.5pmol 时，仅需调整 siRNA 预混液浓度为 0.5μM，转染试剂最低用量为 0.5μL/孔，其他操作维持不变。
（5）RNA-LNP 复合物在室温条件下可稳定存放 8 小时，请勿将 RNA-LNP复合物置于冷冻条件。
（6）转染前后均无需更换细胞培养基，含有血清、双抗的完全培养基对转染效果无明显影响；转染 6h 以后可按细胞常规培养流程，进行换液或其他操作。
附录 A：干细胞 RNA 转染操作说明
1. LNP 干细胞 RNA 转染试剂盒操作流程（推荐用量Ⅰ）
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2. 不同培养容器中每孔试剂用量（推荐用量Ⅰ）
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3. LNP 干细胞 RNA 转染试剂盒操作流程（推荐用量Ⅱ）
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4. 不同培养容器中每孔试剂用量（推荐用量Ⅱ）
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*每孔 LNP常用细胞 RNA 转染试剂推荐用量为 siRNA 转染的最低用量。
附录 B： CRISPR/Cas9 mRNA+gRNA 共转染推荐方案
1. Cas9 mRNA+gRNA 共转染操作流程
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2. 不同培养容器中每孔试剂用量
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LNP RNA Transfection Reagent (Stem Cells) is a novel, ready-to-use transfection reagent developed using lipid nanoparticle (LNP) technology. It uses the unique transmembrane transport and endosomal escape mechanisms of LNPs to deliver RNA efficiently and is broadly suitable for the transfection of different RNA types into stem cells such as MSCs.
Easy to use: No specialized encapsulation equipment is required. A single mixing step enables instantaneous RNA-LNP encapsulation. No incubation is required, and transfection can be performed immediately. There is no need to replace the culture medium after transfection.
Efficient: Encapsulation efficiency can reach 80% or higher for small-volume samples, enabling efficient transfection of stem cells such as MSCs.
Safe: This product is ethanol-free, uses novel biodegradable lipid materials, has extremely low cytotoxicity, and is filtered through a 0.2 μm microporous membrane.
Kit Contents & Storage
S1374075 | Component | 50 μL | 1.5 mL | 3.0 mL | 5.0 mL | Storage |
S1374075A | Stem Cell RNA Transfection Reagent | 50 μL | 1.5 mL | 3.0 mL | 5.0 mL | 2-8 °C |
S1374075B | Buffer | 1 mL | 8 mL | 2 × 8 mL | 3 × 8 mL | 2-8 °C |
Transportation and Storage
Transport under controlled ambient temperature (≤25°C). Store at 2°C~8°C. Do not freeze!
Recommended Applications
LNP RNA Transfection Reagent (Stem Cells) is suitable for in vitro RNA transfection into stem cells such as MSCs. Compatible RNA types include siRNA, miRNA, gRNA, mRNA, saRNA, and circRNA. This product is for research use only.
Precautions
(1) Nuclease-free water is the preferred solvent for RNA stocks. The mRNA stock concentration must be at least 200 ng/μL, the short-chain RNA stock concentration must be at least 100 ng/μL, and the siRNA stock concentration must be at least 10 μM. Other solvents may affect transfection performance; please contact us for advice on specific conditions.
(2) When preparing the RNA premix, use only the Buffer supplied with the kit to dilute the RNA stock. Do not use PBS or culture medium.
(3) Ensure that the RNA premix concentration is 40 ng/μL. When co-transfecting multiple RNA species, such as CRISPR Cas9 mRNA and gRNA, the RNA premix concentration refers to the combined concentration of Cas9 mRNA + gRNA. The recommended ratio of Cas9 mRNA to gRNA is 1:1 to 1:2 (μgmRNA:μggRNA).
(4) The RNA/LNP ratio is a critical factor in LNP encapsulation. To increase or decrease the RNA amount per well, follow the instructions below:
1. For mRNA transfection and mRNA + gRNA co-transfection, increase or decrease the volume of RNA-LNP complexes added per well in proportion to the desired change in mRNA amount.
2. For siRNA transfection, increase the volume of RNA-LNP complexes added per well in proportion to the desired increase in siRNA amount. To decrease the siRNA amount per well, do not reduce the LNP transfection reagent below Recommended Amount II. For a 96-well plate, when reducing the siRNA amount to 0.5 pmol per well, adjust only the siRNA premix concentration to 0.5 μM. The minimum transfection reagent amount is 0.5 μL per well; leave all other steps unchanged.
(5) RNA-LNP complexes are stable at room temperature for 8 h. Do not freeze RNA-LNP complexes.
(6) There is no need to replace the culture medium before or after transfection. Complete medium containing serum and dual antibiotics has no appreciable effect on transfection performance. From 6 h after transfection, medium changes and other procedures may be performed according to the routine cell culture protocol.
Appendix A: Instructions for RNA Transfection into Stem Cells
1. LNP Stem Cell RNA Transfection Kit Workflow (Recommended Amount I)
Figure 1. Stem cell RNA transfection workflow (Recommended Amount I).
2. Per-Well Reagent Amounts for Different Culture Vessels (Recommended Amount I)
RNA cell transfection step | Reagent | 96-well plate | 24-well plate | 6-well plate |
1) Prepare a 40 ng/μL RNA premix | Buffer (example: RNA stock at 1 μg/μL) | 2.4 μL | 12 μL | 60 μL |
RNA | 0.1 μg | 0.5 μg | 2.5 μg | |
2) Prepare RNA-LNP complexes | LNP Stem Cell RNA Transfection Reagent | 2.5 μL | 12.5 μL | 62.5 μL |
RNA premix | 2.5 μL | 12.5 μL | 62.5 μL | |
3) Add to cells | RNA-LNP complexes | 5 μL | 25 μL | 125 μL |
siRNA cell transfection step | Reagent | 96-well plate | 24-well plate | 6-well plate |
1) Prepare a 1 pmol/μL siRNA premix (i.e., 1 μM) | Buffer (example: siRNA stock at 10 μM) | 0.9 μL | 4.5 μL | 22.5 μL |
siRNA | 1 pmol | 5 pmol | 25 pmol | |
2) Prepare siRNA-LNP complexes | LNP Stem Cell RNA Transfection Reagent | 1 μL | 5 μL | 25 μL |
siRNA premix | 1 μL | 5 μL | 25 μL | |
3) Add to cells | siRNA-LNP complexes | 2 μL | 10 μL | 50 μL |
3. LNP Stem Cell RNA Transfection Kit Workflow (Recommended Amount II)
Figure 2. Stem cell RNA transfection workflow (Recommended Amount II).
4. Per-Well Reagent Amounts for Different Culture Vessels (Recommended Amount II)
RNA cell transfection step | Reagent | 96-well plate | 24-well plate | 6-well plate |
1) Prepare a 40 ng/μL RNA premix | Buffer (example: RNA stock at 1 μg/μL) | 2.4 μL | 12 μL | 60 μL |
RNA | 0.1 μg | 0.5 μg | 2.5 μg | |
2) Prepare the LNP premix | Buffer | 1.25 μL | 6.25 μL | 31.25 μL |
LNP Stem Cell RNA Transfection Reagent | 1.25 μL | 6.25 μL | 31.25 μL | |
3) Prepare RNA-LNP complexes | LNP premix | 2.5 μL | 12.5 μL | 62.5 μL |
RNA premix | 2.5 μL | 12.5 μL | 62.5 μL | |
4) Add to cells | RNA-LNP complexes | 5 μL | 25 μL | 125 μL |
siRNA cell transfection step | Reagent | 96-well plate | 24-well plate | 6-well plate |
1) Prepare a 3 pmol/μL siRNA premix (i.e., 1 μM) | Buffer (example: siRNA stock at 10 μM) | 0.9 μL | 4.5 μL | 22.5 μL |
siRNA | 1 pmol | 5 pmol | 25 pmol | |
2) Prepare the LNP premix | Buffer | 0.5 μL | 2.5 μL | 12.5 μL |
LNP Stem Cell RNA Transfection Reagent | 0.5 μL* | 2.5 μL* | 12.5 μL* | |
3) Prepare siRNA-LNP complexes | LNP premix | 1 μL | 5 μL | 25 μL |
siRNA premix | 1 μL | 5 μL | 25 μL | |
4) Add to cells | siRNA-LNP complexes | 2 μL | 10 μL | 50 μL |
* The recommended amount of LNP Common Cell RNA Transfection Reagent per well is the minimum amount for siRNA transfection.
Appendix B: Recommended Protocol for CRISPR/Cas9 mRNA + gRNA Co-Transfection
1. Cas9 mRNA + gRNA Co-Transfection Workflow
Figure 3. Cas9 mRNA + gRNA co-transfection workflow for stem cells.
Cas9 mRNA + gRNA cell transfection step | Reagent | 96-well plate | 24-well plate | 6-well plate |
1) Prepare a 40 ng/μL RNA premix | Buffer | 4.8 μL | 24 μL | 120 μL |
Cas9 mRNA (example: mRNA stock at 1 μg/μL) | 0.1 μg | 0.5 μg | 2.5 μg | |
gRNA (example: gRNA stock at 1 μg/μL) | 0.1 μg | 0.5 μg | 2.5 μg | |
2) Prepare the LNP premix | Buffer | 2.5 μL | 12.5 μL | 62.5 μL |
LNP Stem Cell RNA Transfection Reagent | 2.5 μL | 12.5 μL | 62.5 μL | |
3) Prepare RNA-LNP complexes | LNP premix | 5 μL | 25 μL | 125 μL |
RNA premix | 5 μL | 25 μL | 125 μL | |
4) Add to cells | RNA-LNP complexes | 10 μL | 50 μL | 250 μL |
规格
别名
LNP RNA 转染试剂（干细胞）
规格或纯度
BioReagent, 分子生物学级, 无菌
英文名称
LNP RNA Transfection Reagent (Stem Cells)
应用
Cell Transfection
储存与运输
储存条件
2-8°C储存,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
Store at 2-8℃ (1 Years). Do not freeze.
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ25F0826187 |
分析证书 | S1374075 |
| ZJ25F0826188 |
分析证书 | S1374075 |
技术文档和文章
溶液计算器
常见问题
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本产品应如何储存？
请于2–8 °C条件下保存。请勿冷冻。冷冻可能对本品造成不可逆的损坏。
本产品如何运输？
本产品采用湿冰冷藏运输。收货后请立即拆包，并按上述储存条件保存。
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查看 BioReagent 级别指南 → 查看 Suitable for molecular biology 级别指南 → 查看 Sterile 级别指南 →相关产品
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