计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
精子稀释液 (计数液) 作用原理是将稀释液与精液按一定倍数稀释,充入计数池后在相差显微镜下计数一定体积内精子数,换算求出每毫升精液中精子的数量。该稀释液仅适用于科研领域,不适用于临床诊断或其他用途。
操作步骤(仅供参考):
1、 取洁净小试管,加入精子稀释液,再取液化精液加入稀释液内,充分混匀。
2、 滴入改良Neubauer血细胞计数池内,静置1~2 min,待精子下沉后,以精子头部作为基准进行计数。
3、 如果每个中央方格内精子数<10,应计数所有25个方格内的精子数;如果每个中央方格内精子数在10~40,应计数10个方格内的精子数;如果每个中央方格内精子数在>40,应计数5个方格内的精子数。
4、 计算:精子数=(计数结果/计数中方格数)×(1/计数中方格数)×20×10⁵/ml
注意事项:
1、收集精液前3~7天应避免性生活,收集精液样本后,应在1 h内检查。
2、 出现一次异常结果,应隔1周后复查,反复检查2~3次方能得出比较正确的结果。
3、 如果低倍镜、高倍镜检查均无精子,应将精液离心沉淀后再涂片检查,如果两次均无精子,报告“无精子”。
Sperm Dilution (Counting Solution) works by diluting semen with the diluent at a specific ratio. After loading the mixture into a counting chamber, the number of sperm in a defined volume is counted under a phase-contrast microscope, and the sperm concentration per milliliter of semen is calculated via conversion. This diluent is for research use only and not intended for clinical diagnosis or any other applications.
Operating Procedures (For Reference Only):
1. Take a clean small test tube, add the sperm diluent, then add liquefied semen to the diluent and mix thoroughly.
2. Dispense the mixture onto a modified Neubauer hemocytometer, let it stand for 1–2 minutes until the sperm settle, and count the sperm with the sperm head as the reference standard.
3. If the number of sperm in each central square is < 10**, count the sperm in all 25 central squares; if the number is **10–40** per central square, count the sperm in 10 central squares; if the number is **> 40 per central square, count the sperm in 5 central squares.
4. Calculation: Sperm concentration = (Count result / Number of counted central squares) × (1 / Number of counted central squares) × 20 × 10⁵ /mL
Precautions:
1. Abstain from sexual activity for 3–7 days before semen collection, and examine the semen sample within 1 hour after collection.
2. In case of an abnormal result in a single test, recheck after an interval of 1 week; repeated examinations for 2–3 times are required to obtain a relatively accurate result.
3. If no sperm are observed under both low and high power microscopy, centrifuge the semen for sedimentation and then prepare a smear for recheck. Report "azoospermia" if no sperm are found in two consecutive tests.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | S1511112 | |
| 分析证书 | S1511112 |