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血小板 (Blood platelet) 是哺乳动物血液中的有形成分之一,是从骨髓成熟的巨核细胞胞质裂解脱落下来的具有生物活性的小块胞质。血小板具有特定的形态结构和生化组成,在止血、伤口愈合、炎症反应、血栓形成及器官移植排斥等生理和病理过程中有重要作用。
血小板稀释液 (Platelet Dilution) 是一种能破坏血液中的红细胞,但能保护血小板形态完整的稀释液稀释血液,滴入血细胞计数池,在高倍镜下直接计数血小板数。该血小板稀释液仅用于科研领域,不用于临床诊断及其他用途。
操作步骤(仅供参考):
1、 取小号试管,加入Platelet Dilution 0.38ml。
2、 用洁净干燥微量吸管取毛细血管血20μl,擦去管外余血后加至Platelet Dilution中,轻吸上层清液3次,立即充分混匀。
3、 待完全溶血后再次混匀1 min,取混匀的血小板悬液1滴,充入计数池内,注意产生气泡或外溢,室温静置10-15min,待血小板沉淀。
4、 置于显微镜高倍镜下依次计数中央大方格内四角和中央共五个中方格内血小板数。
计算:
血小板数/L=5个中方格内血小板数×10⁹/L
注意事项:
1、 针刺深度应稍深,拭去第一滴血后,首先采血做血小板计数。操作应迅速,防止血小板聚集。
2、 采血标本后,应在1 h内计数完毕,以免影响结果。
3、 小试管、计数板均应清洁,以免误认细胞。
4、 计数时光线应适中,应注意有折光性的血小板和杂质相区别。
5、 用相位显微镜计数,效果更佳,更准确。
6、 为了您的安全和健康,请穿实验服并戴一次性手套操作。
Platelets are one of the formed elements in the blood of mammals. They are small, biologically active cytoplasmic fragments shed from the cytoplasm of mature megakaryocytes in the bone marrow through lysis. Platelets possess a specific morphological structure and biochemical composition, and play a crucial role in physiological and pathological processes such as hemostasis, wound healing, inflammatory response, thrombosis, and organ transplant rejection.
Platelet Diluent is a type of solution that can lyse red blood cells in the blood while preserving the intact morphology of platelets. It is used to dilute blood samples, which are then loaded into a hemocytometer for the direct counting of platelets under a high-power microscope. This platelet diluent is for research use only and not intended for clinical diagnosis or any other applications.
Operating Procedures (For Reference Only):
1. Take a small test tube and add 0.38 mL of platelet diluent to it.
2. Use a clean, dry micropipette to draw 20 μL of capillary blood. Wipe off excess blood from the outside of the pipette, add the blood to the platelet diluent, aspirate the supernatant gently three times, and mix the solution thoroughly immediately.
3. After complete hemolysis, mix the solution again for 1 minute. Take one drop of the well-mixed platelet suspension and load it into the hemocytometer, taking care to avoid bubble formation or overflow. Let it stand at room temperature for 10–15 minutes to allow platelets to settle completely.
4. Place the hemocytometer under the high-power microscope of a light microscope, and count the number of platelets in five medium squares in total—i.e., the four corner medium squares and the central medium square within the large central counting square—one by one.
Calculation:
Platelet count/L = Number of platelets in 5 medium squares × 10⁹/L
Precautions:
1. The needle puncture should be slightly deep. After wiping off the first drop of blood, collect blood for platelet counting first. Perform all operations rapidly to prevent platelet aggregation.
2. Complete platelet counting within 1 hour after blood collection to avoid interfering with the test results.
3. Ensure the small test tubes and hemocytometers are thoroughly clean to prevent misidentification of foreign particles as platelets.
4. Use moderate light during counting, and take care to distinguish refractive platelets from impurities.
5. Counting with a phase-contrast microscope yields better and more accurate results.
6. For your safety and health, wear a lab coat and disposable gloves during all operations.
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| 批号(Lot Number) | 证书类型 | 货号 |
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| 分析证书 | P1511110 |
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