计算溶液所需的质量、体积或浓度。
中性红-甲基蓝染色液
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
动物细胞内由单层膜包裹的小泡属于液泡系，如溶酶体、高尔基复合体、内质网、吞噬泡、转运泡等，尤以软骨细胞的液泡系发达，植物细胞内亦有类似的液泡系，一般情况下液泡系呈酸性。中性红是一种红色粉末状的弱碱性染料，同时又是一种pH 指示剂，变色范围在 pH 值 6.4～8.0 (由红变黄)。在中性或微碱性环境中，植物的活细胞能大量吸收中性红并向液泡系中排泌，由于细胞核中的核酸以及液泡系均呈酸性，进入其中的中性红解离出大量阳离子而呈现樱桃红色，因此细胞核和液泡系被染成红色，原生质和细胞壁一般不着色。死细胞由于原生质变性凝固，细胞液不能维持在液泡系内，中性红染色后不产生液泡系着色现象，相反中性红的阳离子与带有一定负电荷的原生质及细胞核结合，而使原生质与细胞核染色。甲基蓝是弱酸性染料，能溶于水和酒精，甲基蓝在动植物的制片技术方面应用极广，跟伊红合用能染神经细胞，也是细菌制片中不可缺少的染料，水溶液是原生动物的活体染色剂；甲基蓝极易氧化，因此用其染色后不能长久保存。
中性红-甲基蓝染色液是由 1%中性红和甲基蓝混合而成，用来鉴定细胞的死活，该试剂使活细胞的细胞核和液泡系呈红色，死细胞胞质呈蓝色，胞核呈深蓝色，常用于植物样本的染色。
操作步骤(仅供参考)：
(一)植物组织：
1、取植物根尖，置于载玻片上，刀纵切。
2、滴加中性红-甲基蓝染色液染色 3~8min，吸除染液，滴加一滴 Ringer 液或生理盐水。
3、盖盖玻片，用镊子轻轻下压 (使根尖压扁，便于观察)，普通光镜下观察或拍照。
(二)细胞样品：
1、细胞样品用 PBS 或生理盐水洗涤，2000r/min 离心 10min，沉淀用PBS 重悬。
2、加入等体积中性红-甲基蓝染色液混合染色 2~5min。
3、吸取混合液滴加置载玻片上，盖片，观察或拍照。
染色结果：
活细胞：细胞核和液泡系呈红色
死细胞：胞质呈蓝色，胞核呈深蓝色
注意事项：
1、首次使用本试剂时建议先取 1~2 个样品做预实验。
2、中性红-甲基蓝染色液长期存放会产生少量沉淀，可用细胞筛过滤 (不影响染色效果)。
3、做细胞样本的染色时，可将细胞沉淀与染液混合后涂片观察。
4、样品进行染色时，可以根据染色结果和要求调整时间。
5、中性红染色液时，可以根据染色结果和要求调整时间。
6、为了您的安全和健康，请穿实验服并戴一次性手套操作。
7、本产品仅用于科研，严禁他用。
The vesicles enclosed by a single membrane in animal cells belong to the vacuolar system, such as lysosomes, Golgi complexes, endoplasmic reticula, phagosomes, transport vesicles and so on. The vacuolar system is particularly well-developed in chondrocytes. A similar vacuolar system also exists in plant cells, which is generally acidic under normal conditions. Neutral Red is a weak basic dye in the form of red powder and also a pH indicator with a color change range of pH 6.4–8.0 (turning from red to yellow). In a neutral or slightly alkaline environment, living plant cells can absorb a large amount of Neutral Red and secrete it into the vacuolar system. Since the nucleic acids in the nucleus and the vacuolar system are both acidic, the Neutral Red entering them dissociates a large number of cations and exhibits a cherry red color. Thus, the nucleus and vacuolar system are stained red, while the protoplasm and cell wall are generally unstained. In dead cells, due to the denaturation and coagulation of protoplasm, the cell sap cannot be retained in the vacuolar system, so the vacuolar system will not be stained after Neutral Red treatment. On the contrary, the cations of Neutral Red bind to the protoplasm and cell nucleus with a certain negative charge, resulting in the staining of protoplasm and cell nucleus. Methylene Blue is a weak acidic dye soluble in water and alcohol, and it is widely used in the preparation techniques of animal and plant specimens. Combined with Eosin, it can stain nerve cells, and it is also an indispensable dye in bacterial specimen preparation; its aqueous solution serves as a vital stain for protozoa. Methylene Blue is extremely susceptible to oxidation, so specimens stained with it cannot be preserved for a long time.
Neutral Red-Methyl Blue Stain Solution is a mixture of 1% Neutral Red and Methylene Blue, used to identify cell viability. This reagent stains the nucleus and vacuolar system of living cells red, the cytoplasm of dead cells blue, and the cell nucleus of dead cells dark blue, and is commonly used for the staining of plant samples.
Operating Procedures (For Reference Only):
(I) Plant Tissues
1. Take the plant root tip, place it on a glass slide, and make a longitudinal cut with a knife.
2. Add a drop of Neutral Red-Methylene Blue Staining Solution and stain for 3–8 minutes. Aspirate the staining solution and add one drop of Ringer’s solution or normal saline.
3. Cover with a coverslip and press it gently with forceps (to flatten the root tip for easy observation), then observe or photograph under a light microscope.
(II) Cell Samples
1. Wash the cell samples with PBS or normal saline, centrifuge at 2000 r/min for 10 minutes, and resuspend the pellet in PBS.
2. Add an equal volume of Neutral Red-Methylene Blue Staining Solution, mix well, and stain for 2–5 minutes.
3. Aspirate the mixture, drop it onto a glass slide, cover with a coverslip, and then observe or photograph.
Staining Results:
Live cells: The nucleus and vacuolar system appear red.
Dead cells: The cytoplasm appears blue, and the nucleus appears dark blue.
Precautions:
1. It is recommended to conduct a pre-experiment with 1–2 samples when using this reagent for the first time.
2. A small amount of precipitate may form in the Neutral Red-Methylene Blue Staining Solution after long-term storage, which can be filtered with a cell strainer (no impact on staining effect).
3. For the staining of cell samples, the cell pellet can be mixed with the staining solution before preparing a smear for observation.
4. The staining time can be adjusted according to the staining results and experimental requirements during sample staining.
5. The staining time of Neutral Red Staining Solution can be adjusted according to the staining results and experimental requirements.
6. For your safety and health, wear a lab coat and disposable gloves during operation.
7. This product is for research use only and shall not be used for any other purposes.
规格
别名
中性红-美蓝染色液 | 中红甲基蓝染色液
英文别名
Neutral Red-Methylene Blue Staining
规格或纯度
BioReagent,生物染色剂,用于显微镜
稳定性与储存
Store at room temperature long term (12 months).
英文名称
Neutral Red-Methyl Blue Stain Solution
储存条件
室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0333104 |
分析证书 | N1511064 |
技术文档和文章
溶液计算器
常见问题
什么是「生物染色剂」？
「生物染色剂」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于室温条件下保存。
本产品如何运输？
本产品按常温标准条件运输，无需温控包装。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
需要帮助选择级别？
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Microscopy 级别指南 →相关产品
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