计算溶液所需的质量、体积或浓度。
结晶紫PBS溶液 (0.1%)
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为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
在一般的情况下，细菌易被碱性染料染色，碱性染料电离后显色离子带正电荷，易与带负电荷的被染物结合。由于细菌的等电点在pH 2～5之间，在碱性、中性、弱酸性的环境中细菌均带负电荷，易与带正电荷的染料结合而着色。常用的染料有碱性复红、结晶紫、美蓝等。
结晶紫 (Crystal violet) 又称甲紫，分子式为C₂₅H₃₀N₃Cl·9H₂O，分子量为407.98，CAS号为548-62-9。与甲基绿、中性红、亚甲兰、番红、苏木素、孔雀绿、甲基紫、碱性品红是目前常用的人工合成的碱性染料，可溶于水、乙醇和氯仿，不溶于乙醚，水溶液和乙醇溶液呈深紫色，遇浓硫酸显橙色。用作酸碱指示剂，pH变色范围0.1 (绿色) ～2.0 (蓝色）。在分析化学中用作非水溶液滴定指示剂，生物染色剂，比色测定中的显色剂。结晶紫也是细菌革兰氏染色中的重要染料。
结晶紫 PBS 溶液 (0.1%) 主要由 0.1%结晶紫、PBS Buffer 等组成，多用于细胞染色。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤（仅供参考）：
细胞染色(以6孔板为例)
第1步：细胞固定
1.吸弃培养板中的细胞培养基。
2.用预冷的1× PBS轻轻漂洗细胞1-2次，彻底洗去血清和死细胞碎片。
3.加入固定液(如4%多聚甲醛或甲醇)，确保完全覆盖孔底。
多聚甲醛:室温固定15-20分钟。
甲醇:室温固定10-15分钟。甲醇同时具有固定和通透作用。
4.吸弃固定液。
第2步：PBS洗涤
1.加入足量1×PBS，轻柔摇晃洗涤1-2分钟。
2.吸弃PBS。重复洗涤1次，以彻底去除残留的固定液。
3.(可选)将板子倒扣在吸水纸上轻轻拍干，确保孔内无液体残留。
第3步：结晶紫染色
1.向每个孔中加入适量0.1%结晶紫溶液 (确保完全覆盖细胞层，6孔板约需1ml/孔)。
2.室温下避光孵育。
推荐时间：15-30分钟。具体时间可根据细胞密度和染色强度进行调整。染色时间越长，颜色越深但背景也可能加深。
3.染色结束后，小心吸弃染色液 (注意：结晶紫极易溅洒，污染环境和皮肤)。
第4步：充分洗涤 (关键步骤)
1.用缓慢的细流去离子水或1× PBS轻轻冲洗孔板数次，直至中洗液无色透明。
2.将孔板浸泡在盛满去离子水的大容器中，置于摇床上轻柔振荡10-15分钟，以去除非特异性背景染色。可更换1-2次水，直到水保持清澈。
3.倒掉水，将板子倒扣在干净的吸水纸上，室温下完全风干。
第5步:结果观察
使用普通光学显微镜观察细胞形态和染色情况，细胞呈蓝紫色。
注意事项：
1、 结晶紫对人体有害，请注意适当防护。
2、 第一次使用本试剂时建议先取1～2个样品做预实验。
3、 为了您的安全和健康，请穿实验服并戴一次性手套操作。
4、本产品仅供科研实验，严禁他用。
Under normal circumstances, bacteria are easily stained with basic dyes. The chromogenic ions of basic dyes carry a positive charge after ionization and readily bind to negatively charged stained substances. Since the isoelectric point of bacteria ranges from pH 2 to 5, bacteria carry a negative charge in alkaline, neutral and weakly acidic environments, and are thus prone to combining with positively charged dyes for coloration. Commonly used dyes include basic fuchsin, crystal violet and methylene blue.
Crystal violet, also known as methyl violet, has a molecular formula of C₂₅H₃₀N₃Cl·9H₂O, a molecular weight of 407.98 and a CAS number of 548-62-9. Together with methyl green, neutral red, methylene blue, safranin, hematoxylin, malachite green, methyl violet and basic fuchsin, it is a commonly used synthetic basic dye at present. It is soluble in water, ethanol and chloroform, but insoluble in diethyl ether. Its aqueous and ethanol solutions appear deep purple, and turn orange when exposed to concentrated sulfuric acid. It is used as an acid-base indicator with a pH color change range of 0.1 (green) to 2.0 (blue), a non-aqueous titration indicator in analytical chemistry, a biological stain and a chromogenic agent in colorimetric determination. Crystal violet is also an important dye for Gram staining of bacteria.
Crystalline violet PBS solution (0.1%) is mainly composed of 0.1% crystal violet, PBS Buffer and other components, and is mostly used for cell staining. This reagent is for research use only and not intended for clinical diagnosis or any other applications.
Operating Procedures (For Reference Only):
Cell Staining (Taking 6-well Plate as an Example)
Step 1: Cell Fixation
1. Aspirate and discard the cell culture medium in the culture plate.
2. Gently rinse the cells with pre-cooled 1× PBS for 1–2 times to completely remove serum and dead cell debris.
3. Add a fixative (e.g., 4% paraformaldehyde or methanol) to ensure the bottom of each well is fully covered.
Paraformaldehyde: Fix at room temperature for 15–20 minutes.
Methanol: Fix at room temperature for 10–15 minutes (methanol serves as both a fixative and a permeabilizer).
4. Aspirate and discard the fixative.
Step 2: PBS Washing
1. Add an adequate volume of 1× PBS and wash with gentle shaking for 1–2 minutes.
2. Aspirate and discard the PBS, and repeat the washing step once to completely remove residual fixative.
3. (Optional) Invert the plate and gently pat dry on absorbent paper to ensure no liquid remains in the wells.
Step 3: Crystal Violet Staining
1. Add an appropriate volume of 0.1% crystal violet solution to each well (ensure the cell layer is fully covered; approximately 1 mL per well for a 6-well plate).
2. Incubate at room temperature in the dark.
Recommended incubation time: 15–30 minutes. The specific time can be adjusted according to cell density and staining intensity. Longer staining time results in a deeper color but may also cause a darker background.
3. After staining, carefully aspirate and discard the staining solution (Note: Crystal violet is highly prone to splashing and may contaminate the environment and skin).
Step 4: Thorough Washing (Critical Step)
1. Gently rinse the plate several times with a slow, fine stream of deionized water or 1× PBS until the rinse solution becomes colorless and transparent.
2. Immerse the plate in a large container filled with deionized water and shake gently on a shaker for 10–15 minutes to remove non-specific background staining. The water can be replaced 1–2 times until it remains clear.
3. Pour off the water, invert the plate on clean absorbent paper, and air-dry completely at room temperature.
Step 5: Result Observation
Observe the cell morphology and staining under a regular light microscope. The cells will stain blue-purple.
Precautions:
1. Crystal violet is harmful to the human body; proper protective measures are recommended.
2. It is advised to conduct a pre-experiment with 1–2 samples when using this reagent for the first time.
3. For your safety and health, wear a lab coat and disposable gloves during operation.
4. This product is for research use only and shall not be used for any other purposes.
规格
别名
甲紫 PBS 溶液 | 龙胆紫 PBS 溶液
英文别名
Methyl Violet-PBS Solution | Gentian Violet-PBS Solution
规格或纯度
BioReagent,生物染色剂,用于显微镜,0.1%
稳定性与储存
Store at room temperature long term (12 months). Store in the dark.
英文名称
Crystalline violet PBS solution (0.1%)
储存条件
避光,室温
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0939616 |
分析证书 | C1511055 |
| ZJ26F0333050 |
分析证书 | C1511055 |
溶液计算器
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