计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
甲苯胺蓝 (Toluidine Blue O) 中的阳离子有染色作用,组织细胞的酸性物质与其中的阳离子相结合而被染色。甲苯胺蓝还含有两个助色团,能促使染料产生电离成盐类,帮助发色团对组织产生染色力,使切片上的组织细胞着色,可染细胞核使之呈蓝色。临床上,经常用甲苯胺蓝对软骨细胞和肥大细胞进行染色。
甲苯胺蓝染色液 (细胞专用) 适用于细胞内酸性黏液多糖染色,尤其适用于含硫的酸性黏液物质染色,染色后酸性黏多糖呈红色。
操作步骤(仅供参考):
1、细胞涂片后,滴加甲苯胺蓝染色液 (细胞专用) 染色5min。
2、滴加等量蒸馏水于涂片上混匀,染色15min。
3、水洗、晾干、镜检。
染色结果:
细胞内酸性黏液多糖呈红色
注意事项:
1、为了证实染色效果,可选用慢性粒细胞白血病血片 (嗜酸性粒细胞) 或再生障碍性贫血骨髓涂片 (组织嗜酸性粒细胞) 作对照。
2、为了您的安全和健康,请穿实验服并戴一次性手套操作。
3、 本产品仅供科研使用,严禁它用。
The cations in Toluidine Blue O exert the staining effect, and acidic substances in tissue cells combine with these cations to be stained. Toluidine Blue O also contains two auxochromes that promote the ionization of the dye to form salts, enhance the staining power of chromophores on tissues, and thus stain the tissue cells on sections-specifically, it can stain cell nuclei blue. Clinically, Toluidine Blue O is frequently used to stain chondrocytes and mast cells.
Toluidine Blue O Stain Solution (For Cells) is suitable for staining acidic mucopolysaccharides in cells, especially for staining sulfur-containing acidic mucous substances, which appear red after staining.
Operating Procedures (For Reference Only):
1. After preparing the cell smear, add Toluidine Blue Staining Solution (Cell-Specific) dropwise and stain for 5 minutes.
2. Add an equal volume of distilled water to the smear, mix well, and stain for another 15 minutes.
3. Rinse with water, air-dry the smear, and examine under a microscope.
Staining Result:
Acidic mucopolysaccharides in cells appear red.
Precautions:
1. To verify the staining effect, blood smears from chronic myeloid leukemia (eosinophils) or bone marrow smears from aplastic anemia (tissue eosinophils) can be used as controls.
2. For your safety and health, wear a lab coat and disposable gloves during operation.
3. This product is for research use only and shall not be used for any other purposes.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | T1511051 | |
| 分析证书 | T1511051 |
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