计算溶液所需的质量、体积或浓度。
红细胞稀释液 (计数液)
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
红细胞也称红血球，在常规化验英文常缩写成RBC，是血液中数量最多的一种血细胞，同时也是脊椎动物体内通过血液运送氧气的最主要的媒介，同时还具有免疫功能。哺乳动物成熟的红细胞是无核的，这意味着它们失去了DNA。红细胞也没有线粒体，它们通过葡萄糖合成能量。运输氧气，也运输一部分二氧化碳。运输二氧化碳时呈暗紫色，运输氧气时呈鲜红色，同时还具有免疫功能。
红细胞稀释液 (RBC Dilution) 作用原理是等渗稀释液将血液按一定倍数稀释，充入计数池后显微镜下计数一定体积内红细胞数，换算求出每升血液中红细胞的数量。该红细胞稀释液仅用于科研领域，不用于临床诊断。
自备材料：
1、新鲜全血或EDTA抗凝全血
2、微量吸管
3、血球计数板
4、显微镜
操作步骤（仅供参考）：
1、 取中号试管，加 RBC Dilution 2 ml。
2、 用洁净干燥微量吸管取末梢血或抗凝血 10 μl，擦去管外余血后加至 RBC Dilution 底部，再轻吸上层清液清洗吸管2-3次，立即混匀。
3、 用干净微量吸管将红细胞悬液充入细胞计数板的细胞池，注意产生气泡或外溢，室温静置1～3 min。
4、 置于显微镜高倍镜下依次计数中央大方格内四角和正中共5个中方格内的红细胞。压线细胞按“数上不数下，数左不数右”的原则进行计数。
计算：
红细胞数/L=5 个中方格内红细胞数×5×10×200×10⁶=5 个中方格内红细胞数×10¹⁰
| ×5 | 5个中方格换算成1个大方格 |
| ×10 | 1个大方格容积为 0.1 μl，换算成 1.0 μl |
| ×200 | 血液的实际稀释倍数应为201倍，按200倍计算 |
| ×10⁶ | 由 1 μl换算成 1 L |
注意事项：
1、 采血时不能过于挤压，针刺深度应适当。
2、 不应以血红蛋白浓度来折算红细胞数。
3、 在参考范围内，两次红细胞计数相差不应超过5%。
4、 为了您的安全和健康，请穿实验服并戴一次性手套操作。
Red blood cells, also known as erythrocytes and commonly abbreviated as RBC in routine laboratory tests, are the most numerous type of blood cell in the blood. They serve as the primary medium for oxygen transport in the blood of vertebrates and also possess immune functions. Mature red blood cells in mammals are anucleate, meaning they lack DNA. They also have no mitochondria and produce energy through glucose metabolism. In addition to transporting oxygen, they carry a portion of carbon dioxide—appearing dark purple when transporting carbon dioxide and bright red when transporting oxygen—and they also have immune functions.
Principle of Action of Red Blood Cell Diluent (RBC Dilution), An isotonic diluent is used to dilute blood at a specific ratio. The diluted blood is then loaded into a hemocytometer, and the number of red blood cells in a defined volume is counted under a microscope. The red blood cell concentration per liter of blood is subsequently calculated via conversion. This RBC diluent is for research use only and not intended for clinical diagnosis.
Self-Prepared Materials:
1. Fresh whole blood or EDTA-anticoagulated whole blood
2. Micropipettes
3. Hemocytometers
4. Microscopes
Operating Procedures (For Reference Only):
1. Take a medium-sized test tube and add 2 mL of RBC Dilution to it.
2. Use a clean, dry micropipette to draw 10 μL of peripheral blood or anticoagulated blood. Wipe off excess blood from the outside of the pipette, insert the pipette to the bottom of the RBC Dilution, then gently aspirate the supernatant to rinse the pipette 2–3 times and mix the solution thoroughly immediately.
3. Use a clean micropipette to load the red blood cell suspension into the counting chamber of the hemocytometer, taking care to avoid bubble formation or overflow. Let it stand at room temperature for 1–3 minutes.
4. Place the hemocytometer under the high-power microscope and count the red blood cells in five medium squares in total—the four corner medium squares and the central medium square within the large central counting square—one by one. For cells overlapping the grid lines, follow the counting principle of "count cells touching the top and left lines, not the bottom and right lines".
Calculation:
RBC count/L= Number of red blood cells in 5 medium squares×5×10×200×10⁶=Number of red blood cells in 5 medium squares×10¹⁰
| ×5 | Convert the count from 5 medium squares to 1 large square |
| ×10 | Convert the volume of 1 large square (0.1 μL) to 1.0 μL |
| ×200 | The actual blood dilution ratio is 201-fold, calculated as 200-fold for convenience |
| ×10⁶ | Convert from 1 μL to 1 L |
Precautions:
1. Do not over-squeeze the puncture site during blood collection, and ensure an appropriate needle puncture depth.
2. Do not convert hemoglobin concentration to red blood cell count.
3. Within the reference range, the difference between two replicate red blood cell counts should not exceed 5%.
4. For your safety and health, wear a lab coat and disposable gloves during all operations.
规格
别名
红细胞计数液
英文别名
Red Blood Cell Dilution | Erythrocyte Dilution | RBC dilution
规格或纯度
BioReagent,生物染色剂,用于显微镜
稳定性与储存
Store at room temperature long term (12 months).
英文名称
Red Blood Cell Dilution (Counting Solution)
储存条件
室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0333073 |
分析证书 | M1511108 |
| ZJ26F0333074 |
分析证书 | M1511108 |
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