计算溶液所需的质量、体积或浓度。
精子形态学快速染色液 (Diff-Quick法)
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Diff-Quik染色是在Wright染色基础上改良而来的一种快速染色方法，是细胞学检查中常用的染色方法之一，染液是采用世界卫生组织(WHO)推荐的快速染色方法而配制，与WrightStain类似都是利用Romanowsky Stain技术原理改良而来的，染色结果与瑞氏染色液也极其相似，但迪夫快速染色所需的时间极短，一般90s以内即可完成染色。
精子形态的评估可以采用Diff-Quik染色液。精子形态学快速染色液 (Diff-Quik法) 属于WHO推荐的精子形态学染色，其染色原理是精子及细胞内不同等电点的蛋白质在相同的酸度下带不同的电荷，能选择性地结合相应的染料而着色；嗜酸性蛋白质解离的氨基带正电荷，能与带负电荷的酸性染料(如嗜酸性氧杂蒽、伊红)结合而被染成红色；嗜碱性蛋白质解离的羧基带负电荷，能与带正电荷的碱性染料(嗜碱性硫氮杂苯、亚甲蓝)结合而被染成蓝色；嗜中性蛋白质解离的带正电荷的氨基和带负电荷的羧基相等，同时结合相等的酸性染料和碱性染料而呈紫红色，但因解离电荷相等，故着色。较弱。该染色液主要用于精子(细胞)形态的评估，含固定液，非常适合用于批量浸染，且背景清晰无沉渣，该染色液仅用于科研领域，不宜用于临床诊断或其他用途。
产品组分及储存条件：
| S1511533 | Component | 3×20mL | 3×100mL | Storage |
| S1511533A | Diff‑Quik 固定液 | 20mL | 100mL | RT |
| S1511533B | Diff‑Quik 染色液 Ⅰ | 20mL | 100mL | RT. Store in the dark. |
| S1511533C | Diff‑Quik 染色液 Ⅱ | 20mL | 100mL | RT. Store in the dark. |
自备材料：
载玻片、蒸馏水、显微镜
操作步骤(仅供参考)：
1、制备涂片：彻底清洗2张载玻片，再用70%的乙醇洗涤，晾干。滴加5～20μl于玻片上，用第2张载玻片的边缘在清洁载玻片表面拖拉一滴精液，制成涂片。如果精子密度过高，可用生理盐水适当稀释。
2、Diff‑Quik 固定液或自然干燥，固定15～20s。
3、将玻片直立于吸水纸上以去除多余的液体。
4、玻片入Diff‑Quik 染色液Ⅰ染色10～20s，将玻片直立于吸水纸上以去除多余的液体。
5、玻片入Diff‑Quik 染色液Ⅱ染色5～10s，将玻片直立于吸水纸上以去除多余的液体。
6、流水浸洗10～15次以去除多余的染液。
7、将玻片直立于吸水纸上以去除多余的水分，并使其完全干燥，显微镜下观察。
染色结果：
精子头部顶体区：淡蓝色
精子顶体区后区：深蓝色
精子中断：可能为淡红色
精子尾部：蓝色或淡红色不等
注意事项：
1、如果精子密度＞20×10⁶/ml，应取5μl；如果精子密度＜20×10⁶/ml，应取5～20μl。
2、Diff‑Quik 固定液是精子Diff-Quik染色法的与用固定液，如需大量可用甲醇替代。
3、精子样本应新鲜，精子涂片应厚薄均匀，以免影响染色效果，如果精子密度过高，可采用生理盐水适当稀释。
4、涂片染色中请勿先去除染液或直接对涂片用力冲洗。不能先倒掉染液，以免染料沉着于涂片上。
5、染色液可重复使用，但不能多次重复，若有沉淀物应过滤后使用。
6、染色过深可用甲醇或酒精适当脱色，最好不复染。
7、如果染色过深或过浅，应调整染色时间或工作液浓度。
8、pH值对染色有一定影响，载玻片应清洁、无酸碱污染，以免影响染色效果
9、为了您的安全和健康，请穿实验服并戴一次性手套操作。
10、本产品仅用于科研，严禁他用。
Diff-Quik staining is a rapid staining method improved on the basis of Wright staining, and is one of the commonly used staining methods in cytological examination. The staining solution is prepared according to the rapid staining method recommended by the World Health Organization (WHO). Similar to Wright Stain, it is also modified based on the principle of Romanowsky Stain. The staining results are very similar to those of Wright stain, but Diff-Quik rapid staining requires an extremely short time, and can generally be completed within 90 seconds. Diff-Quik staining solution can be used for the evaluation of sperm morphology. Rapid Sperm Morphology Staining Solution (Diff-Quik method) is a sperm morphology staining method recommended by WHO. Its staining principle is that proteins with different isoelectric points in sperm and cells carry different charges at the same acidity and can selectively bind to corresponding dyes for coloration. Amino groups dissociated from eosinophilic proteins are positively charged and can bind to negatively charged acidic dyes (such as eosinophilic xanthene, eosin) and be stained red. Carboxyl groups dissociated from basophilic proteins are negatively charged and can bind to positively charged basic dyes (such as basophilic thiazine, methylene blue) and be stained blue. Neutrophilic proteins have equal numbers of positively charged amino groups and negatively charged carboxyl groups after dissociation; they bind equal amounts of acidic and basic dyes simultaneously and appear purplish red, but the staining intensity is weak because the net charge is zero. This staining solution is mainly used for the evaluation of sperm (cell) morphology. It contains fixative, is very suitable for batch staining, and features a clear background without sediment. This product is for research use only and is not suitable for clinical diagnosis or other purposes.
Product Components and Storage Conditions:
| S1511533 | Component | 3×20mL | 3×100mL | Storage |
| S1511533A | Diff-Quik Fixative | 20mL | 100mL | RT |
| S1511533B | Diff-QuikⅠ | 20mL | 100mL | RT. Store in the dark. |
| S1511533C | Diff-QuikⅡ | 20mL | 100mL | RT. Store in the dark. |
Materials Required (User-supplied):
Glass slides, distilled water, microscope
Protocol (For Reference Only):
1. Smear preparation: Clean two glass slides thoroughly, wash with 70% ethanol and air-dry. Place 5–20 μl of sample on the slide. Drag the droplet of semen across the clean slide surface using the edge of a second slide to prepare a smear. If sperm density is excessively high, dilute appropriately with physiological saline.
2. Fix in Diff‑Quik Fixative or by air drying for 15–20 seconds.
3. Stand the slide vertically on absorbent paper to remove excess fluid.
4. Stain the slide in Diff‑Quik Stain I for 10–20 seconds, then stand vertically on absorbent paper to remove excess stain.
5. Stain the slide in Diff‑Quik Stain II for 5–10 seconds, then stand vertically on absorbent paper to remove excess stain.
6. Dip and rinse 10–15 times in running water to remove excess dye.
7. Stand the slide vertically on absorbent paper to remove excess moisture and allow to dry completely. Examine under a microscope.
Staining Results:
Acrosomal region of sperm head: Pale blue
Post-acrosomal region of sperm head: Dark blue
Midpiece of sperm: Pale red (may be)
Sperm tail: Blue or pale red (variable)
Precautions:
1. If sperm density > 20×10⁶/mL, use 5 μL; if sperm density < 20×10⁶/mL, use 5–20 μL.
2. Diff‑Quik Fixative is the dedicated fixative for sperm Diff‑Quik staining. Methanol may be used as an alternative if a large volume is required.
3. Sperm samples should be fresh, and smears should be of uniform thickness to avoid affecting staining results. If sperm density is excessively high, dilute appropriately with physiological saline.
4. Do not remove the staining solution first or rinse the smear forcefully during staining. Do not pour off the stain first, to prevent dye precipitation on the smear.
5. The staining solution can be reused, but not repeatedly. If sediment is present, filter before use.
6. If overstained, appropriate decolorization may be performed with methanol or ethanol; restaining is not recommended.
7. If staining is too intense or too faint, adjust the staining time or working solution concentration.
8. pH affects staining. Slides must be clean and free of acid or alkali contamination.
9. For your safety and health, wear lab coat and disposable gloves during operation.
10. This product is for research use only. Strictly prohibited for other purposes.
规格
别名
精子活体染色试剂盒 | 精子迪夫快速染色试剂盒
英文别名
Sperm Morphology Stain
规格或纯度
BioReagent,生物染色剂,用于显微镜
稳定性与储存
Store at room temperature long term (12 months). Store in the dark.
英文名称
Sperm morphology rapid staining solution (Diff-Quick method)
储存条件
避光,室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0434274 |
分析证书 | S1511533 |
技术文档和文章
溶液计算器
常见问题
什么是「生物染色剂」？
「生物染色剂」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于室温、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
本产品如何运输？
本产品按常温标准条件运输，无需温控包装。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
需要帮助选择级别？
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Microscopy 级别指南 →产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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