计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
尿沉渣的检测有多种方法,如定量检查法、离心沉淀检查法、染色检查法等。Sternheimer-Malbin染色液属于染色尿沉渣检查法的一种,由结晶紫、沙黄等组成,染色后红细胞呈淡紫色,白细胞呈橙红色。该试剂仅用于科研领域,不宜用于临床诊断或其他用途。
操作步骤(仅供参考):
1、取0.2ml沉渣,滴加1滴Sternheime-Malbin染色液混合。
2、覆盖盖玻片或充入计数板,放置,镜检。
染色结果:
红细胞呈淡紫色,多形核白细胞的细胞核呈橙红色,细胞质内可见颗粒,细胞管型呈深紫色,透明管型呈粉红色或淡紫色。
注意事项:
1、应准备干净、干燥采尿杯,一般情况下留取中段尿。
2、在为了获得细胞沉淀的离心的过程中,对于特殊细胞,如果细胞沉淀不充分,可以适当提高离心力或延迟离心时间。
3、为了保持尿沉渣中细胞成分维持原来的形态特征,要求迅速送检,2h内检查完毕。
4、为了您的安全和健康,请穿实验服并戴一次性手套操作。
5、本产品仅供科研使用,严禁它用。
There are various methods for the examination of urinary sediment, such as quantitative examination, centrifugal sedimentation examination, staining examination, etc. Sternheimer-Malbin Stain is one type of staining method for urinary sediment examination, composed of crystal violet, safranine, etc. After staining, erythrocytes appear pale purple, and leukocytes appear orange-red. This reagent is for research use only and is not suitable for clinical diagnosis or other purposes.
Protocol (for reference only):
1. Take 0.2 mL of sediment, add 1 drop of Sternheimer-Malbin Stain, and mix.
2. Cover with a coverslip or fill into a counting chamber, let stand, and examine microscopically.
Staining Results:
Erythrocytes appear pale purple; nuclei of polymorphonuclear leukocytes appear orange-red with visible granules in the cytoplasm; cellular casts appear dark purple; hyaline casts appear pink or pale purple.
Precautions:
1. Use clean, dry urine collection cups. Generally, midstream urine should be collected.
2. During centrifugation to obtain cell sediment, for special cells, if sedimentation is insufficient, centrifugal force may be appropriately increased or centrifugation time extended.
3. To preserve the original morphological characteristics of cellular components in urinary sediment, prompt submission is required, and examination should be completed within 2 hours.
4. For your safety and health, please wear a lab coat and disposable gloves during operation.
5. This product is for research use only, strictly prohibited for other purposes.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | U1511467 | |
| 分析证书 | U1511467 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Microscopy 级别指南 →