计算溶液所需的质量、体积或浓度。
软骨染色液 (阿利新蓝法, pH2.5)
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光,室温。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
阿利新蓝(Alcian)又称爱先蓝或阿尔辛蓝等，是一种类铜钛花青共轭染料，最初用于纺织纤维染色。这种阳离子染料与酸性基团结合，也即阿尔辛蓝与组织内含有的阴离子基团如羧基和硫酸根形成不溶性复合物。阿利新蓝由中央含铜的酞菁环与四个异硫脲基通过硫醚键相连而成，该异硫脲基呈中度碱性，使阿利新蓝带阳离子。pH 值为 2.5时，组织内的羧基电离，带有一个负电荷，与阿利新蓝中的阳离子形成盐键，使带有羧基的酸性黏液物质(硫酸黏蛋白和唾液黏蛋白)染色。pH 值为 1.0 时，组织内的硫酸根电离，带有一个负电荷，与阿利新蓝中的阳离子形成盐键，使带有硫酸根的组织(如硫酸黏液物质)染色。
软骨染色液 (阿利新蓝法, pH2.5) 利用染液的不同 pH 值可区分粘液物质的类属，pH 为1时，羧基(-COOH) 不着色，硫酸基(-OSO₃H) 着色，pH 为 2.5 时，羧基染色良好而硫酸粘液着色不佳，使硫酸黏蛋白和唾液黏蛋白着色。本试剂仅适用于科研领域，不适用于临床诊断或其他用途。
操作步骤(仅供参考)：
1、常规脱钙，二甲苯脱蜡，通过梯度乙醇后，入蒸馏水再水化。
2、入Alcian酸化液浸泡。
3、入Alcian染色液染色。
4、流水冲洗。
5、梯度乙醇脱水，二甲苯透明，中性树胶封片。
染色结果：
酸性黏液物质(羟基)：蓝色
细胞核：淡蓝色
注意事项：
1、固定液采用中性福尔马林。
2、若要选择性鉴别硫酸黏蛋白和蛋白多糖，应使用pH值低(pH=1.0)的阿利新蓝，即调pH值为1.0染色程序与pH=2.5的阿利新蓝操作相同，染色时间应相应延长。
3、已开封试剂应在开封后6个月内使用完，每次用后应及时拧紧瓶盖，以免挥发或变质。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
5、本产品仅用于科研，严禁他用。
Alcian Blue, also known as Alcian, is a copper phthalocyanine-based conjugated dye originally used for dyeing textile fibers. This cationic dye binds to acidic groups; that is, Alcian Blue forms insoluble complexes with anionic groups in tissues such as carboxyl groups and sulfate groups. Alcian Blue consists of a central copper-containing phthalocyanine ring linked to four isothiouronium groups via thioether bonds. The isothiouronium groups are moderately basic, rendering Alcian Blue cationic. At pH 2.5, carboxyl groups in tissues are ionized and carry a negative charge, forming salt linkages with the cationic groups in Alcian Blue, thus staining acidic mucinous substances (sulfated mucins and sialomucins) that contain carboxyl groups. At pH 1.0, sulfate groups in tissues are ionized and carry a negative charge, forming salt linkages with the cationic groups in Alcian Blue, thus staining tissues containing sulfate groups (e.g., sulfated mucins).
Cartilage Staining Solution (Alcian Blue Method, pH 2.5) differentiates types of mucinous substances by using different pH values. At pH 1.0, carboxyl groups (-COOH) are not stained, while sulfate groups (-OSO₃H) are stained. At pH 2.5, carboxyl groups are stained well but sulfated mucins are poorly stained, allowing differentiation between sulfated mucins and sialomucins. This reagent is for research use only and is not suitable for clinical diagnosis or other purposes.
Protocol (For Reference Only):
1. Routine decalcification, dewax with xylene, pass through graded ethanol, then rehydrate in distilled water.
2. Immerse in Alcian Acid Solution.
3. Stain in Alcian Staining Solution.
4. Rinse in running water.
5. Dehydrate in graded ethanol, clear in xylene, and mount with neutral balsam.
Staining Results:
Acid mucins (hydroxyl groups): Blue
Cell nuclei: Pale blue
Precautions:
1. Neutral formalin is used as the fixative.
2. For selective identification of sulfated mucins and proteoglycans, Alcian Blue with a low pH value (pH=1.0) should be used. The staining procedure at pH=1.0 is the same as that for Alcian Blue at pH=2.5, and the staining time should be prolonged accordingly.
3. Opened reagents should be used within 6 months after opening. Recap tightly immediately after each use to prevent evaporation or deterioration.
4. For your safety and health, please wear lab coat and disposable gloves during operation.
5. This product is for research use only. Strictly prohibited for other purposes.
规格
别名
软骨染色液
英文别名
Cartilage Stain (Alisin Blue Method, pH2.5)
规格或纯度
BioReagent,生物染色剂,用于显微镜
英文名称
Cartilage Staining Solution (Alcian Blue, pH2.5)
应用
Bone Tissue Staining, 细胞染色
储存与运输
储存条件
2-8°C储存,避光,室温
运输条件
低温运输
稳定性与储存
各组分在相应的储存条件下保质期1年。
组分与储存
| C1511664 | Component | 2×50mL | Storage |
| C1511664A | Alcian 酸化液 | 50mL | RT |
| C1511664B | Alcian 染色液 (pH2.5) | 50mL | 2-8℃. Store in the dark. |
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 I2607490 |
分析证书 | C1511664 |
| E2620663 |
分析证书 | C1511664 |
| ZJ26F0434147 |
分析证书 | C1511664 |
技术文档和文章
生物染色剂
目录： 阿拉丁试剂的规格、级别和纯度
Principles and methods of smear staining, microbiological staining, and fundamental dye systems
目录： 技术文章
Principles and Applications of Safranin O-Fast Green Staining in Cartilage Histological Evaluation
目录： 技术文章
Application Differences Among Safranin O, Alcian Blue, and Toluidine Blue in Cartilage Matrix Staining
目录： 技术文章
溶液计算器
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