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Diff-Quik染色是在Wright染色基础上经过发展和改良而得到的一种快速染色方法,通常在细胞学检查用此方法进行染色,染液是采用世界卫生组织(WHO)建议的快速染色方法而配制,与Wright Stain相似都是利用Romanowsky Stain技术原理改良而来的,染色结果与瑞氏染色液也极其相似,但迪夫快速染色所需的时间非常短,一般在90 s以内即可完成染色。
迪夫快速染色液不含有固定液,主要用于血细胞涂片、骨髓涂片、阴道分泌物涂片、脱落细胞涂片等染色,非常适合用于批量浸染,背景清晰没有沉渣。该染色液仅用于科研领域,不宜用于临床诊断或其他用途。
产品组分及储存条件:
| D1511557 | Component | 2×100mL | 2×500mL | Storage |
| D1511557A | Diff‑Quik 染色液 Ⅰ | 100mL | 500mL | RT. Store in the dark. |
| D1511557B | Diff‑Quik 染色液 Ⅱ | 100mL | 500mL | RT. Store in the dark. |
操作步骤(仅供参考):
1、 常规方法制备血液涂片或骨髓涂片,自然干燥或酒精灯火焰处理后,甲醇固定20-30s。
2、 入Diff‑Quik 染色液Ⅰ染色5-10s。
3、 入Diff‑Quik 染色液Ⅱ染色5-10s。
4、 水洗后立即趁湿在显微镜下观察。
5、 将观察后认为有价值的片子用二甲苯透明,中性树胶封固、保存。
染色结果:
细胞核、白细胞:深蓝色
基质、淋巴细胞:紫色
细胞质、红细胞:粉红色
注意事项:
1、血液涂片或骨髓涂片应厚薄均匀,以免影响染色效果。
2、血细胞涂片染色要求新鲜全血或EDTA抗凝血。
3、骨髓涂片染色要求制备好的涂片在空气中快速摇动或扇干,以防止细胞皱缩变形或因空气潮湿而溶血,不能用高温或火烤来干燥涂片。
4、阴道分泌物涂片染色要求新鲜标本涂片后,尽快以火焰或酒精固定,以免细胞变形。
5、如果染色过深或过浅,应调整染色时间或工作液浓度。
6、pH值对染色有一定影响,载玻片应清洁、无酸碱污染,以免影响染色效果。
7、为了您的安全和健康,请穿实验服并戴一次性手套操作。
8、本产品仅供科研实验,严禁他用。
Diff-Quik staining is a rapid staining method developed and improved on the basis of Wright staining. It is commonly used for staining in cytological examinations. The staining solution is formulated according to the rapid staining method recommended by the World Health Organization (WHO). Similar to Wright Stain, it is modified based on the technical principle of Romanowsky Stain, and its staining results are also highly similar to those of Wright staining solution. However, Diff-Quik rapid staining requires a very short time, and staining can generally be completed within 90 seconds.
Diff-Quik Stain does not contain fixative solution. It is mainly used for staining blood smears, bone marrow smears, vaginal secretion smears, exfoliative cell smears, etc. It is very suitable for batch dip staining, with a clear background and no sediment. This staining solution is for research use only and is not suitable for clinical diagnosis or other purposes.
Product Components and Storage Conditions:
| D1511557 | Component | 2×100mL | 2×500mL | Storage |
| D1511557A | Diff-QuikⅠ | 100mL | 500mL | RT. Store in the dark. |
| D1511557B | Diff-QuikⅡ | 100mL | 500mL | RT. Store in the dark. |
Protocol (For Reference Only):
1. Prepare blood smears or bone marrow smears using routine methods. Allow to air-dry or briefly pass over an alcohol lamp flame, then fix with methanol for 20–30 seconds.
2. Stain in Diff‑Quik Stain Solution I for 5–10 seconds.
3. Stain in Diff‑Quik Stain Solution II for 5–10 seconds.
4. Rinse with water and examine immediately under a microscope while wet.
5. Clear valuable slides with xylene, mount and preserve with neutral balsam.
Staining Results:
Nuclei, leukocytes: Dark blue
Matrix, lymphocytes: Purple
Cytoplasm, erythrocytes: Pink
Precautions:
1. Blood or bone marrow smears should be of uniform thickness to avoid affecting staining results.
2. Staining of blood cell smears requires fresh whole blood or EDTA anticoagulated blood.
3. For bone marrow smears, shake or fan rapidly in air after preparation to prevent cell shrinkage, deformation or hemolysis due to high humidity. Do not dry smears with high heat or open flame.
4. For vaginal discharge smears, fix fresh specimens promptly by flame or alcohol after smearing to prevent cell deformation.
5. If staining is too intense or too faint, adjust staining time or working solution concentration accordingly.
6. pH affects staining; slides must be clean and free of acid or alkali contamination.
7. For your safety and health, wear a lab coat and disposable gloves during operation.
8. This product is for research use only, strictly prohibited for other purposes.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | D1511557 | |
| 分析证书 | D1511557 |
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