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为什么选择此级别

EnzymoPure™ 级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 66 篇同行评审文献引用。

概述

Tyrosinase (Polyphenol oxidase) is a bifunctional, copper-containing oxidase having catecholase and cresolase activity. It is responsible for browning reactions through the phylogenetic scale. The enzyme has a molecular weight of 128 kDa. It is a tetramer containing four atoms of copper per molecule and two binding sites for aromatic compounds including phenolic substrates. There is a distinct binding site for oxygen. The optimum pH range is 6-7.

Tyrosinase Assay

Method

Polyphenol oxidase oxidizes tyrosine to dihydroxyphenylalanine which in turn is oxidized to o-quinone. The latter is accompanied by an increase in absorbance at 280 nm. The rate of increase is proportional to enzyme concentration and linear during a period of 5-10 minutes after an initial lag. One unit causes a change in absorbance at 280 nm of 0.001 per minute at 25°C, pH 6.5 under the specified conditions.

Reagents

0.5 M Potassium phosphate buffer, pH 6.5

0.001 M L-Tyrosine

Enzyme

Dissolve at a concentration of 1 mg/ml in reagent grade water. Dilute further in reagent grade water to a concentration of 200-400 units/ml.

Procedure

Adjust the spectrophotometer to 280 nm and 25°C. Pipette into each cuvette as follows:

0.5M Phosphate buffer, pH 6.5 1.0 ml

0.001M L-Tyrosine 1.0 ml

Reagent grade water 0.9 ml

Oxygenate this reaction mixture by bubbling oxygen into cuvettes through a capillary tube for 4-5 minutes. Transfer cuvettes to the spectrophotometer and record A280 for 4-5 minutes to achieve temperature equilibration and to establish blank rate, if any. Add 0.1 ml of appropriately diluted enzyme and record A280 for 10-12 minutes. Determine ΔA280 from the linear portion of the curve. A non-linear "lag" of 2-3 minutes can be expected.

Calculation

规格

产品名称

酪氨酸酶来源于菌菇

别名

多酚氧化酶 | 儿茶酚氧化酶 | 单酚单加氧酶

英文别名

Polyphenol Oxidase | Catechol Oxidase | Monophenol Monooxygenase | Monophenol | dihydroxyphenylalanine:oxygen oxidoreductase

规格或纯度

EnzymoPure™, ≥500 units/mg dry weight

生化机理

Copper-containing oxidase that catalyzes both the o-hydroxylation of monophenols and the subsequent oxidation of the resulting o-diphenols into reactive o-quinones, which evolve spontaneously to produce intermediates, which associate in dark brown pigments. Involved in the initial step of melanin synthesis. Melanins constitute a mechanism of defense and resistance to stress such as UV radiations, free radicals, gamma rays, dehydratation and extreme temperatures, and contribute to the fungal cell-wall resistance against hydrolytic enzymes in avoiding cellular lysis. Fungal pigments are also involved in the formation and stability of spores (By similarity).

生物活性

≥500 units/mg dry weight

种属

双孢蘑菇(Agaricus bisporus)

Accession #

Q00024; O42713; C7FF04; C7FF05

来源

天然

预测分子量

128 kDa

CAS编号和信息

9002-10-2

酶学委员会编号

EC 1.14.18.1

分子类型

酶

储存与运输

物理形态

冻干(Lyophilized)

浓度

≥500 units/mg dry weight

储存条件

-20°C储存

运输条件

超低温运输

单位定义

One unit causes an increase in the absorbance at 280 nm of 0.001 per minute at 25°C, pH 6.5, using L-tyrosine as substrate.

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

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✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

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产品规格和应用的快速参考摘要。

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🔬 规格说明书

该级别的完整质量属性和验收标准。

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高级数据

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

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批号(Lot Number)	证书类型	货号
G2615650	分析证书	T128536
E2627606	分析证书	T128536
E2627608	分析证书	T128536
G2613458	分析证书	T128536
G2613457	分析证书	T128536
E2506337	分析证书	T128536
E2506338	分析证书	T128536
L2418485	分析证书	T128536
L2418484	分析证书	T128536
K2401642	分析证书	T128536
K2401643	分析证书	T128536
G2416541	分析证书	T128536
G2416542	分析证书	T128536
G2416540	分析证书	T128536
E2428378	分析证书	T128536
E2428377	分析证书	T128536
L2304373	分析证书	T128536
L2304376	分析证书	T128536
H2318746	分析证书	T128536
H2318747	分析证书	T128536
J2225671	分析证书	T128536
J2225698	分析证书	T128536
G2416043	分析证书	T128536
A2411063	分析证书	T128536

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引用文献

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41. Xinqing Shi, Jiaman Liu, Wanqin Zhang, Shinian Zeng, Fan Lin, Haining Wang, Bo Teng. (2024) Mechanism of the wine pomace tannin in hyperpigmentation inhibition: Impact on signaling pathways, cell proliferation, and tyrosinase activity. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:39038577] [10.1016/j.ijbiomac.2024.134052]
42. Yuanting Chen, Linlin Xu, Sheng Zhao, Chenfang Miao, Yuyuan Chen, Zhenzhen Wang, Feng Feng, Mingrui Lin, Shaohuang Weng. (2024) One-pot hydrothermal synthesis of silicon, nitrogen co-doped carbon dots for enhancing enzyme activity of acid phosphatase (ACP) to dopamine and for cell imaging. TALANTA, [PMID:38917549] [10.1016/j.talanta.2024.126451]
43. Xiaohong Yu, Mengli Peng, Xiaocheng Liu, Yueling Shang, Dujun Wang, Wenbin Jin, Fengwei Li. (2024) Physicochemical properties, moisture retention, and biological activities of polysaccharides from Panax notoginseng separated by fractional precipitation. CHEMISTRY & BIODIVERSITY, [PMID:39363708] [10.1002/cbdv.202402002]
44. Yan-Xia Li, He Ma, Le Shi, Zhong-Fei Zhang, Yu-Meng Wu, Chao Yan, Yan Chen, Yong-Ming Lu. (2025) Purification, characterization and protective effects on UVB-induced photoaging in zebrafish of Pleurotus citrinopileatus polysaccharide PCP-2. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, [PMID:40156221] [10.1002/jsfa.14247]
45. Shi-Huan Guo, Zimeng Zhang, Xi Zuo, Sitong Jiao, Jingyao Sun, Yahao Yang, Yahong Yuan, Tianli Yue. (2025) Synthesis and evaluation of the antioxidant, anti-tyrosinase and anti-browning activities of N-caffeoyl amino acid methyl esters. LWT-FOOD SCIENCE AND TECHNOLOGY, [10.1016/j.lwt.2025.117409]
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