计算溶液所需的质量、体积或浓度。
Verhöeff弹力纤维染色液 (伊红复染)
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
结缔组织狭义上是指其含有的三种纤维：胶原纤维、网状纤维、弹力纤维，弹力纤维(ElasticFiber)主要分布于人体的动脉壁、肺泡壁、皮肤，新鲜时呈黄色，折光性强。弹力纤维染色可以显示皮肤组织中弹力纤维的变化，如弹力纤维痣、皮肤环状肉芽肿、硬度病等；显示与判定心内膜及动脉的病变，观察某些病变中是否伴有弹力纤维的增生或破坏；还可鉴别肿瘤组织成分如弹力纤维瘤，经弹力纤维染色后，可清晰见到瘤体内弹力纤维球。常用的弹力纤维染色法有Gomori醛品红法、间苯二酚碱性品红法、地衣红法、维多利亚蓝法、铁碘苏木素法等。
Verhöeff弹力纤维染色液(伊红复染)属于铁碘苏木素染色法，其染色原理是弹力蛋白与前弹力蛋白纤维可通过二硫键高度交联，经碘氧化后，部分二硫键转化为阴离子的硫酸衍生物，该衍生物呈强嗜碱性，对染液中的碱性染料苏木素有选择性吸收。本产品采用改良的伊红复染法，染色后粗纤维着色较深，细纤维染色效果稍差，细胞核和弹力纤维呈(蓝)黑色，其他组织呈红色，对比清晰。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
产品组分及储存条件：
| V1518609 | Component | 3×50mL | Storage |
| V1518609A | Verhoeff 染液 A | 30mL | RT |
| V1518609B | Verhoeff 染液 B | 12mL | RT |
| V1518609C | Verhoeff 染液 C | 12mL | RT. Store in the dark. |
| V1518609D | Verhoeff 分化液 | 50mL | RT |
| V1518609E | 伊红染色液 | 50mL | RT |
临用前，按A:B:C=5:2:2混合即为Verhoeff染液，2~ 3h内使用。
自备材料：
二甲苯或脱蜡透明液脱蜡、系列乙醇、中性树胶
操作步骤(仅供参考)：
1、石蜡切片二甲苯或脱蜡透明液脱蜡至水。
2、切片入配制好的Verhöeff染液的染缸中浸染10~30min，自来水快速冲洗。
3、用Verhöeff分化液分化至弹力纤维呈黑色，背景呈灰色，自来水快速冲洗。
4、95%乙醇快速冲洗脱碘。
5、用伊红染色液复染25~60s，吸干过多的染色液。
6、无水乙醇快速脱水，二甲苯或脱蜡透明液透明，中性树胶封固。
染色结果：
弹力纤维、细胞核：黑色或蓝黑色
红细胞：棕黄色
其他组织：不同程度的红色
注意事项：
1、大多数固定液均可用于本染色液中的切片固定。
2、应注意Verhöeff分化液分化程度的控制，细纤维易因分化过度导致不染色。
3、温的自来水冲洗可以增强纤维着色程度。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
In a narrow sense, connective tissue refers to the three types of fibers it contains: collagen fibers, reticular fibers, and elastic fibers. Elastic fibers are mainly distributed in the arterial walls, alveolar walls, and skin of the human body. They appear yellow when fresh and have strong refraction. Elastic fiber staining can demonstrate changes in elastic fibers in skin tissue, such as elastic nevus, granuloma annulare, and scleroderma; it can reveal and evaluate endocardial and arterial lesions, and observe whether some lesions are accompanied by hyperplasia or destruction of elastic fibers. It can also identify tumor components such as elastofibroma, in which elastic fiber globules can be clearly seen after elastic fiber staining. Common elastic fiber staining methods include Gomori Aldehyde Fuchsin, Resorcinol-Fuchsin, Orcein, Victoria Blue, Iron-Iodine Hematoxylin, and others.
Verhöeff Elastic Fiber Staining Solution (Eosin Counterstain) belongs to the iron-iodine hematoxylin staining method. Its staining principle is that elastin and proelastin fibers are highly cross-linked via disulfide bonds. After oxidation by iodine, part of the disulfide bonds is converted into anionic sulfuric acid derivatives, which are strongly basophilic and selectively absorb the basic dye hematoxylin in the staining solution. This product uses a modified eosin counterstaining method. After staining, coarse fibers are deeply stained, while fine fibers show slightly weaker staining. Nuclei and elastic fibers appear (blue) black, and other tissues appear red, with clear contrast. This reagent is for research use only and not for clinical diagnosis or other purposes.
Product Components and Storage Conditions:
| V1518609 | Component | 3×50mL | Storage |
| V1518609A | Verhöeff Staining Solution A | 30mL | RT |
| V1518609B | Verhöeff Staining Solution B | 12mL | RT |
| V1518609C | Verhöeff Staining Solution C | 12mL | RT. Store in the dark. |
| V1518609D | Verhöeff Differentiation Solution | 50mL | RT |
| V1518609E | Eosin Staining Solution | 50mL | RT |
Immediately before use, mix A:B:C at a ratio of 5:2:2 to prepare Verhoeff Staining Solution. Use within 2–3 hours.
Materials Required (User-supplied):
Xylene or eco-friendly dewaxing and clearing agent, graded ethanol, neutral balsam
Protocol (For Reference Only):
1. Deparaffinize paraffin sections with xylene or dewaxing clearing agent and hydrate to water.
2. Stain sections in freshly prepared Verhöeff Staining Solution for 10–30 min, then rinse rapidly with tap water.
3. Differentiate in Verhöeff Differentiation Solution until elastic fibers appear black and the background gray; rinse rapidly with tap water.
4. Rinse quickly in 95% ethanol to remove iodine.
5. Counterstain with Orange G Staining Solution for 25–60 s, then blot excess stain.
6. Dehydrate rapidly in absolute ethanol, clear with xylene or dewaxing clearing agent, and mount with neutral balsam.
Staining Results:
Elastic fibers and nuclei: Black or blue-black
Erythrocytes: Brownish yellow
Other tissues: Red in varying degrees
Precautions:
1. Most fixatives are suitable for fixation of sections used with this staining solution.
2. Pay close attention to the degree of differentiation with Verhöeff Differentiation Solution; fine fibers may fail to stain if over-differentiated.
3. Rinsing with warm tap water can enhance staining intensity of fibers.
4. For your safety and health, wear a lab coat and disposable gloves during operation.
规格
别名
Verhöeff弹力纤维染色液
英文别名
Elastic Fiber Stain (Eosin Red)
规格或纯度
BioReagent,生物染色剂,用于显微镜
稳定性与储存
Store at room temperature long term (12 months). Store in the dark.
英文名称
Verhöeff Elastic Fiber Staining Solution (Eosin Counterstain)
储存条件
避光,室温
运输条件
常规运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0535356 |
分析证书 | V1518609 |
技术文档和文章
Principles and methods of smear staining, microbiological staining, and fundamental dye systems
目录： 技术文章
Application Differences Among Highman, Bennhold, Puchtler, and Modified Methods in Congo Red Staining for Amyloid
目录： 技术文章
Identification of Tissue Components, Method Selection, and Result Interpretation in Connective Tissue Staining
目录： 技术文章
How Does Dynamic ECM Remodeling in the Tumor Microenvironment Regulate Tumor Immune Responses?
目录： 技术文章
Selection of Special Staining Methods and Their Applications in Pathological Diagnosis: Fibers, Carbohydrates, Lipids, Pigments, and Pathogen Staining
目录： 技术文章
溶液计算器
常见问题
什么是「生物染色剂」？
「生物染色剂」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于室温、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
本产品如何运输？
本产品按常温标准条件运输，无需温控包装。
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每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
选择需要帮助选择级别？
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Microscopy 级别指南 →产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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