细胞定位:Secreted.
序列相似性:Belongs to the glycosyl hydrolase 13 family.
α-淀粉酶被用于水解α-连接多糖(如淀粉和糖原)的α键。本产品来自于解淀粉芽孢杆菌(Bacillus amyloliquefaciens)并以液体形式提供。α-淀粉酶已用于各种植物研究,如拟南芥(Arabidopsis)的代谢研究。 来自解淀粉芽孢杆菌(Bacillus amyloliquefaciens )的α-淀粉酶已用于水解甘薯支链淀粉,以鉴定簇结构。该酶已被用于将西米棕榈淀粉水解为还原糖,然后将其用于酿酒酵母(Saccharomyces cerevisiae)FNCC 3012的乙醇发酵。该酶能够催化胶凝蜡质玉米淀粉的淀粉分解,产生还原糖。
生化机理
内淀粉酶能够随机水解直链淀粉和支链淀粉中的α-(1→4)-糖苷键。分解产物是具有不同链长度的低聚糖和糊精。该酶在高温下(70−90°C)仍具有活性。
来自解淀粉芽孢杆菌(B.amyloliquefaciens)的α-淀粉酶的pH范围为5.5至6.5,在pH 5.9时具有单一最大值。在低于pH 5.0的条件下,酶迅速失活。
α-淀粉酶是一种内淀粉酶,能够随机水解直链淀粉和支链淀粉中的α-(1,4)-糖苷键。分解产物是具有不同链长度的低聚糖和糊精。该酶在70-90°C的高温下具有最佳活性。
α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. This product is from Bacillus amyloliquefaciens and is supplied as a liquid. α-Amylase has been used in various plant studies, such as metabolism studies in Arabidopsis. α-Amylase from Bacillus amyloliquefaciens has been used to hydrolyze sweetpotato amylopectin to identify cluster structure. The enzyme has been used to hydrolyze sago palm starch to reducing sugars, which are then used for ethanol fermentation by Saccharomyces cerevisiae FNCC 3012. The enzyme catalyzes amylolysis of gelatinised waxy maize starch to produce reducing sugars.
Biochem/physiol Actions
α-Amylase is an endoamylase that randomly hydrolyzes the α-(1,4) glucan linkages in amylose and amylopectin. The breakdown products are oligosaccharides and dextrins of varying chain length. This enzyme works best at high temperatures, in the range of 70-90 ° Celcius.
An endoamylase that randomly hydrolyzes α-(1→4)-glycosidic linkages in amylose and amylopectin. The breakdown products are oligosaccharides and dextrins of varying chain length. This enzyme is active at high temperatures (70−90 °C).
The pH range for the α-amylase of B.amyloliquefaciens is 5.5 to 6.5, with a single maximum at 5.9. The activity is rapidly inactivated below pH 5.0.