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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
乙酰辅酶 A（Acetyl-CoA）广泛存在于动物、植物、微生物和培养细胞中。是生物体能源物质代谢过程中产生的一种重要的中间代谢产物。在体内能源物质代谢中是一个枢纽性的物质。糖、脂肪、蛋白质三大营养物质通过乙酰辅酶A汇聚成一条共同的代谢通路--三羧酸循环和氧化磷酸化，经过这条通路彻底氧化生成二氧化碳和水，释放能量用于 ATP 合成。此外，乙酰辅酶 A 是合成脂肪酸，酮体，胆固醇及其衍生物等生理活性物质的前体物质。 本试剂盒检测原理是苹果酸脱氢酶可催化苹果酸和 NAD 生成草酰乙酸和 NADH。柠檬酸合酶可催化乙酰辅酶 A 和草酰乙酸生成柠檬酸和辅酶 A。利用苹果酸脱氢酶和柠檬酸合酶的偶联反应，乙酰辅酶A含量和NADH的生成速率成正比，NADH 在 340 nm 处有特征吸收峰，通过检测 340 nm 吸光值的计算既可得到乙酰辅酶A含量。
Acetyl-CoA (Acetyl-Coenzyme A) is widely present in animals, plants, microorganisms, and cultured cells. It is a crucial intermediate metabolite produced during the metabolism of energy substances in living organisms and serves as a pivotal hub in the body's energy metabolism. The three major nutrients—carbohydrates, fats, and proteins—converge via Acetyl-CoA into a common metabolic pathway: the tricarboxylic acid cycle and oxidative phosphorylation. Through this pathway, they are completely oxidized to carbon dioxide and water, releasing energy for ATP synthesis. Furthermore, Acetyl-CoA acts as a precursor for the synthesis of physiologically active substances such as fatty acids, ketone bodies, cholesterol, and their derivatives. The detection principle of this kit is that malate dehydrogenase catalyzes the conversion of malate and NAD to oxaloacetate and NADH. Citrate synthase catalyzes the conversion of Acetyl-CoA and oxaloacetate to citrate and coenzyme A. Utilizing the coupled reactions of malate dehydrogenase and citrate synthase, the amount of Acetyl-CoA is proportional to the generation rate of NADH. NADH has a characteristic absorption peak at 340 nm. By measuring the absorbance at 340 nm, the content of Acetyl-CoA can be calculated.
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