计算溶液所需的质量、体积或浓度。
AB-PAS染色试剂盒
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
阿利新蓝 (又称阿尔辛蓝、爱先蓝等) 和PAS技术联合使用可鉴别同一组织切片中的中性黏蛋白和酸性黏蛋白。这种技术也常用作广泛检测黏蛋白的手段，切片先经标准阿利新蓝 (pH=2.5) 染色再使用PAS技术。阿利新蓝可将唾液黏蛋白、硫黏蛋白和蛋白多糖染成蓝色。PAS技术可将中性黏蛋白染成深红或红紫色，同时将既含中性黏蛋白又含酸性黏蛋白的组织和细胞染成深浅不同的紫色，这是由于阿利新蓝与Schiff试剂结合并发生反应。上述染色常可出现在含有中性黏蛋白和唾液黏蛋白的小肠杯状细胞中。阿利新蓝的染色原理在于是类铜钛花青染料，这种阳离子染料与酸性基团结合，也即阿利新蓝与组织内含有的阴离子基团如羧基和硫酸根形成不溶性复合物。分子中带正电荷的盐键与酸性黏多糖物质中带负电荷的酸性基团结合形成不溶性的复合物而呈蓝色，再与PAS进行复合染色，就能显示三种不同黏液物质成分。
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使用说明：
1、组织经固定，常规脱蜡至水，蒸馏水洗1-2min。
2、入3%醋酸溶液 3min。
3、不经水洗，入阿尔新蓝染色液中染色15-30min。
4、蒸馏水洗3次，每次1-2min。
5、入过碘酸溶液，氧化5-10min。
6、流水稍冲洗，蒸馏水洗2次。
7、入Schiff试剂中浸染10-20min。
8、亚硫酸液冲洗3次，每次2min。
9、流水冲洗2min，蒸馏水洗2次。
10、入苏木素染色液，染核1-2min。
11、用酸性分化液分化2-5s，充分水洗。
12、流水冲洗返蓝。
13、常规无水乙醇脱水。二甲苯透明，中性树胶封固。
注意事项：
1、切片脱蜡应尽量干净，否则影响染色效果。
2、过碘酸氧化时间不宜过久，氧化时的温度以18-22°C最佳。
3、阿尔新蓝染色液、过碘酸溶液、Schiff试剂、苏木素溶液均应置于2-8°C密闭保存，使用时避免接触过多阳光和空气。使用前最好提前30min取出恢复至室温，避光暗处使用。
4、酸性分化液应经常更换新液，分化时间应该依据切片厚薄、组织类别和酸性分化液的新旧而定，另外分化后自来水冲洗时间应该足够。
5、切片在过碘酸溶液和Schiff试剂中作用时间非常重要，该依据切片厚薄、组织类别等决定。
6、用苏木素染色液复染细胞核时应淡染，以免影响阳性物质AB的观察。目的是防止胞浆或黏蛋白着色而掩盖阿利新蓝的颜色。
7、阿尔新蓝-PAS联合技术的染色顺序可影响最终结果。PAS技术在阿尔辛蓝染色之前时，中性黏蛋白和糖原可染成紫色。与此相反，阿利新蓝染色在PAS技术之前时，则可将这些物质染成预期的紫红色。
8、亚硫酸液配制：1mol/L稀盐酸7.5mL，10%偏重亚硫酸钠7.5mL，蒸馏水130mL，混合配制而成。
9、为了您的安全和健康，请穿实验服并戴一次性手套操作。
染色结果：
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备注：含有中性黏蛋白和酸性黏蛋白的细胞或组织可染成不同程度的蓝紫色至紫色。
Alcian blue (also known as Alcian blue, Alcian blue, etc.) and PAS technique can be used together to identify neutral mucin and acidic mucin in the same tissue section. This technique is also commonly used as a means to extensively detect mucin. The section is first stained with standard Alcian blue (pH=2.5) and then subjected to the PAS technique. Alcian blue stains sialomucin, sulfomucin, and proteoglycans blue. The PAS technique stains neutral mucin deep red or reddish-purple, while staining tissues and cells containing both neutral and acidic mucin in varying shades of purple. This is due to the combination and reaction of Alcian blue with Schiff reagent. The above staining often appears in small intestinal goblet cells containing neutral mucin and sialomucin. The staining principle of Alcian blue lies in its being a copper phthalocyanine dye. This cationic dye binds to acidic groups, meaning Alcian blue forms insoluble complexes with anionic groups in tissues, such as carboxyl and sulfate groups. The positively charged salt bonds in the molecule combine with the negatively charged acidic groups in acidic mucopolysaccharides to form insoluble complexes that appear blue. When combined with PAS for composite staining, it can display three different mucus substance components.
| A774824 | Component | 6×50 mL | 6×100 mL | Storage |
| A774824A | 3% Acetic Acid Solution | 50 mL | 100 mL | RT. |
| A774824B | Alcian Blue Staining Solution | 50 mL | 100 mL | 2-8℃. Store in the dark |
| A774824C | Periodic Acid Solution | 50 mL | 100 mL | 2-8℃. Store in the dark |
| A774824D | Schiff Reagent | 50 mL | 100 mL | 2-8℃. Store in the dark |
| A774824E | Hematoxylin Staining Solution | 50 mL | 100 mL | 2-8℃. Store in the dark |
| A774824F | Acidic Ethanol Differentiation Solution | 50 mL | 100 mL | RT. |
Instructions for Use
Fix the tissue, deparaffinize routinely to water, and rinse with distilled water for 1–2 min.
Place in 3% acetic acid solution for 3 min.
Without water rinsing, place in Alcian blue staining solution for 15–30 min.
Rinse with distilled water three times, each for 1–2 min.
Place in periodic acid solution for oxidation for 5–10 min.
Rinse slightly under running water, then rinse with distilled water twice.
Immerse in Schiff reagent for 10–20 min.
Rinse with sulfite solution three times, each for 2 min.
Rinse under running water for 2 min, then rinse with distilled water twice.
Place in hematoxylin staining solution for nuclear staining for 1–2 min.
Differentiate with acidic differentiation solution for 2–5 s, then rinse thoroughly with water.
Rinse under running water until blue color returns.
Dehydrate routinely with absolute ethanol. Clear with xylene and mount with neutral balsam.
Precautions
Deparaffinization of sections should be as thorough as possible; otherwise, staining results may be affected.
Oxidation time with periodic acid should not be too long. The optimal temperature for oxidation is 18–22°C.
Alcian blue staining solution, periodic acid solution, Schiff reagent, and hematoxylin solution should be stored tightly sealed at 2–8°C. Avoid excessive exposure to sunlight and air during use. It is best to take them out 30 minutes before use to allow them to return to room temperature, and use them in a dark place away from light.
Acidic differentiation solution should be replaced frequently with fresh solution. Differentiation time should be determined based on section thickness, tissue type, and the freshness of the acidic differentiation solution. Additionally, the rinsing time with tap water after differentiation should be sufficient.
The action time of sections in periodic acid solution and Schiff reagent is crucial and should be determined based on section thickness, tissue type, etc.
When counterstaining cell nuclei with hematoxylin staining solution, staining should be light to avoid affecting the observation of AB-positive substances. The purpose is to prevent cytoplasmic or mucin staining from masking the color of Alcian blue.
The staining sequence of the Alcian blue-PAS combined technique can affect the final results. When the PAS technique is performed before Alcian blue staining, neutral mucin and glycogen may stain purple. Conversely, when Alcian blue staining is performed before the PAS technique, these substances can be stained in the expected reddish-purple color.
Preparation of sulfite solution: Mix 7.5 mL of 1 mol/L dilute hydrochloric acid, 7.5 mL of 10% sodium metabisulfite, and 130 mL of distilled water.
For your safety and health, please wear a lab coat and disposable gloves during operation.
Staining Results
| Glycogen, neutral mucin, various glycoproteins | Reddish-purple |
| Acidic mucin (sulfomucin and sialomucin) | Blue |
| Proteoglycans and hyaluronic acid | Blue |
Note: Cells or tissues containing both neutral and acidic mucin may stain in varying shades from bluish-purple to purple.
规格
别名
阿利辛蓝过碘酸雪夫染色液 | 糖原染色液 | 阿利新蓝PAS联合染色法 | 阿利新蓝-过碘酸雪夫 (AB-PAS) 染色液
英文别名
Alcian blue-Periodic Acid-Schiff Staining Kit | Alcian blue-Periodic Acid-Schiff Staining Solution
规格或纯度
BioReagent, 用于显微镜, 生物染色剂
英文名称
AB-PAS staining kit
敏感性
Light-sensitive
储存与运输
储存条件
2-8°C储存,避光
运输条件
低温运输
稳定性与储存
Store at 2-8℃ long term (12 months). Store in the dark.
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 G2623253 |
分析证书 | A774824 |
| ZJ26F0635945 |
分析证书 | A774824 |
| ZJ25F0421075 |
分析证书 | A774824 |
| ZJ25F0421074 |
分析证书 | A774824 |
| ZJ25F0421073 |
分析证书 | A774824 |
| ZJ25F0421071 |
分析证书 | A774824 |
| ZJ25F0421072 |
分析证书 | A774824 |
| ZJ25F0421070 |
分析证书 | A774824 |
技术文档和文章
生物染色剂
目录： 阿拉丁试剂的规格、级别和纯度
Histological and Cytological Section Staining: Principles and Methodological Essentials for Routine Pathology Stains and Structural Special Stains
目录： 技术文章
Principles, Classification, and Clinical Laboratory Applications of Hematologic Cytochemical Staining
目录： 技术文章
Morphological Basis, Method Workflow, and Result Interpretation of Urinary Sediment Microscopy
目录： 技术文章
Selection of Special Staining Methods and Their Applications in Pathological Diagnosis: Fibers, Carbohydrates, Lipids, Pigments, and Pathogen Staining
目录： 技术文章
线虫与虫卵形态学观察方法
目录： 技术文章
溶液计算器
常见问题
什么是「生物染色剂」？
「生物染色剂」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于2–8 °C、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
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本产品采用湿冰冷藏运输。收货后请立即拆包，并按上述储存条件保存。
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每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
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