As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
宿主种属
兔(Rabbit)
特异性
CST3
种属反应性
人(Human),小鼠(Mouse)
免疫原
Recombinant protein of human Cystatin C
阳性对照
WB: K562, HeLa, Hep G2, A549 and NIH/3T3 cell lysates. IHC: Human Cerebrum tissue, Human Kidney tissue. IF/ICC: HeLa cells.
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
图片
Recombinant Cystatin C Antibody (Ab008364) - Western Blot All lanes: Recombinant Cystatin C Antibody (Ab008364) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 16 kDa Observed band size: 15 kDa Exposure time: 3.9 s
Recombinant Cystatin C Antibody (Ab008364) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant Cystatin C Antibody (Ab008364). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant Cystatin C Antibody (Ab008364) - IF/ICC
IF analysis of Recombinant Cystatin C Antibody (Ab008364) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Cystatin C Antibody (Ab008364) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
ZJ24F0303232
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