ExactAb™,已验证,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human A-Raf Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/200
Protein Function
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.
Recombinant A-Raf Antibody (Ab086857) - Western Blot
All lanes: Recombinant A-Raf Antibody (Ab086857) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 80.0 s
Recombinant A-Raf Antibody (Ab086857) - Western Blot
All lanes: Recombinant A-Raf Antibody (Ab086857) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 100.0 s
Recombinant A-Raf Antibody (Ab086857) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant A-Raf Antibody (Ab086857). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant A-Raf Antibody (Ab086857) - IF/ICC
IF analysis of Recombinant A-Raf Antibody (Ab086857) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant A-Raf Antibody (Ab086857) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
1/500-1/1000
1/50 - 1/100
1/200
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0607007 |
分析证书 | Ab086857 |
| ZJ24F0607008 |
分析证书 | Ab086857 |
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