ExactAb™,已验证,无载体 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human AASS Antibody, Polyclonal, could be used for WB, ICC, IF and so on.
Application:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
Protein Relevance:
Bifunctional enzyme that catalyzes the first two steps in lysine degradation. The N-terminal and the C-terminal contain lysine-ketoglutarate reductase and saccharopine dehydrogenase activity, respectively.
AASS Antibody (Ab086911) - Western Blot
All lanes: AASS Antibody (Ab086911) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDa
Exposure time: 120.0 s
AASS Antibody (Ab086911) - IF/ICC
Immunofluorescent analysis of AASS staining in VB2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with AASS Antibody (Ab086911) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
1/50-1/200
1/500-1/1000
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1012478 |
分析证书 | Ab086911 |
| ZJ24F1012479 |
分析证书 | Ab086911 |
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