Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
宿主种属
兔(Rabbit)
特异性
Acetyl Coenzyme A Carboxylase
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human Acetyl CoA carboxylase (AA 370-430).
阳性对照
WB: HeLa and Jurkat cell lysates. IHC: Human kidney tissue. IF/ICC: HeLa cells.
Acetyl Coenzyme A Carboxylase Antibody (Ab087139) - Western Blot All lanes: Acetyl Coenzyme A Carboxylase Antibody (Ab087139) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 266 kDa Observed band size: 250 kDa Exposure time: 24.6 s
Acetyl Coenzyme A Carboxylase Antibody (Ab087139) - IHC Immunohistochemistry analysis of paraffin-embedded Human kidney using Acetyl Coenzyme A Carboxylase Antibody (Ab087139). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Acetyl Coenzyme A Carboxylase Antibody (Ab087139) - IF/ICC IF analysis of Acetyl Coenzyme A Carboxylase Antibody (Ab087139) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Acetyl Coenzyme A Carboxylase Antibody (Ab087139) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).