Rabbit anti Human ACOX1/AOX Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function: Catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs. Isoform 2 is twice as active as isoform 1 against 16-hydroxy-palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl-CoA.
Recombinant ACOX1/AOX Antibody (Ab087209) - Western Blot All lanes: Recombinant ACOX1/AOX Antibody (Ab087209) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 75 kDa Observed band size: 49, 72 kDa Exposure time: 5.6 s The expression profile observed is consistent with what has been described in the literature (PMID: 8798738 and PMID: 17255948).
Recombinant ACOX1/AOX Antibody (Ab087209) - IF/ICC Immunocytochemistry analysis of ACOX1 (green) in HeLa using Recombinant ACOX1/AOX Antibody (Ab087209) and DAPI (blue).