ExactAb™,已验证,无载体 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human ADPGK Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/100-1/200
IF/ICC: 1/100-1/500
Protein Function
Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency.
ADPGK Antibody (Ab087591) - Western Blot
All lanes: ADPGK Antibody (Ab087591) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 53 kDa
Observed band size: 42, 52 kDa
ADPGK Antibody (Ab087591) recognizes different isoforms of ADPGK.
Exposure time: 200.0 s
ADPGK Antibody (Ab087591) - IHC
Immunohistochemical analysis of ADPGK staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with ADPGK Antibody (Ab087591) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ADPGK Antibody (Ab087591) - IF/ICC
Immunofluorescent analysis of ADPGK staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ADPGK Antibody (Ab087591) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
1/500-1/1000
1/100-1/500
1/200
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0809727 |
分析证书 | Ab087591 |
| ZJ24F0809728 |
分析证书 | Ab087591 |
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