AGBL2 Antibody (Ab087661) - Western Blot All lanes: AGBL2 Antibody (Ab087661) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 104 kDa Observed band size: 94 kDa Exposure time: 8.5 s
AGBL2 Antibody (Ab087661) - Western Blot Western blot analysis of lysates from HUVEC cells, using AGBL2 Antibody (Ab087661). The lane on the right is blocked with the synthesized peptide.
AGBL2 Antibody (Ab087661) - IHC Immunohistochemical analysis of paraffin-embedded human heart tissue, using AGBL2 Antibody (Ab087661). The picture on the right is blocked with the synthesized peptide.
AGBL2 Antibody (Ab087661) - IF/ICC IF analysis of AGBL2 Antibody (Ab087661) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with AGBL2 Antibody (Ab087661) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab138205) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).