Rabbit anti Human ALEX1 Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on.
规格
产品名称
ALEX1 Antibody
别名
ARM重复X连锁蛋白ARMCX1抗体
英文别名
ARMCX 1 AD032 | ALEX1 | ARM protein lost in epithelial cancers on chromosome X 1 | Arm protein lost in epithelial cancers X chromosome 1 | Armadillo repeat containing X linked 1 | Armadillo repeat containing X linked protein 1 | Armadillo repeat-containin
规格或纯度
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
宿主种属
兔(Rabbit)
特异性
ARMCX1
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse),猴(Monkey)
免疫原
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ARMCX1
阳性对照
WB: Rat kidney lysates. IHC: Human brain formalin tissue. IF/ICC: HeLa cells
ALEX1 Antibody (Ab088136) - Western Blot All lanes: ALEX1 Antibody (Ab088136) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/10000 dilution Predicted band size: 49 kDa Observed band size: 46 kDa Exposure time: 60.0 s
ALEX1 Antibody (Ab088136) - IHC Immunohistochemical analysis of ARMCX1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with ALEX1 Antibody (Ab088136) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ALEX1 Antibody (Ab088136) - IF/ICC Immunofluorescent analysis of ARMCX1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ALEX1 Antibody (Ab088136) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.