There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996).
宿主种属
兔(Rabbit)
特异性
Alkaline Phosphatase
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human Alkaline Phosphatase (AA 1-61).
阳性对照
WB: Mouse kidney, Rat kidney, HeLa, Hep G2 and JAR lysates. IHC: Human liver tissue. ICC/IF: HeLa cells.
Recombinant Alkaline Phosphatase Antibody (Ab088188) - Western Blot All lanes: Recombinant Alkaline Phosphatase Antibody (Ab088188) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 57 kDa Observed band size: 75 kDa Exposure time: 60.0 s
Recombinant Alkaline Phosphatase Antibody (Ab088188) - Western Blot All lanes: Recombinant Alkaline Phosphatase Antibody (Ab088188) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 57 kDa Observed band size: 75 kDa Exposure time: 30.9 s
Recombinant Alkaline Phosphatase Antibody (Ab088188) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant Alkaline Phosphatase Antibody (Ab088188).
Recombinant Alkaline Phosphatase Antibody (Ab088188) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant Alkaline Phosphatase Antibody (Ab088188) at 1/50 dilution.
Recombinant Alkaline Phosphatase Antibody (Ab088188) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant Alkaline Phosphatase Antibody (Ab088188) at 1/150 dilution.
Recombinant Alkaline Phosphatase Antibody (Ab088188) - IHC Immunohistochemical analysis of paraffin-embedded human liver, using Recombinant Alkaline Phosphatase Antibody (Ab088188).
IF analysis of Recombinant Alkaline Phosphatase Antibody (Ab088188) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Alkaline Phosphatase Antibody (Ab088188) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
ZJ24F0910184
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