Mouse anti Human Alpha smooth muscle Actin Antibody, Monoclonal (F1), could be used for WB, IHC and so on. Application WB:0.001-2 μg/mL IHC: 5-20 µg/mL Protein Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Recombinant ACTA2 expressed in E.coli (AA 167-377).
阳性对照
WB: NIH/3T3, Mouse skeletal and Mouse heart lysates; IHC: Human Ovarian cancer tissues, Human Rectum tissue, Rat Skeletal muscle tissues, Rat Lung tissue, Mouse Pancreas tissues, Mouse Spleen tissues.
偶联
Unconjugated
克隆类型
单克隆抗体
克隆号
F1
Format
Whole IgG
亚型
Mouse IgG1
轻链亚型
kappa
纯化方法
Protein A + Protein G purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 50% Glycerol, 0.02% Sodium azide, pH7.4
防腐剂
0.02% Sodium azide
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4℃ 短期储存（1-2 周）。-20℃长期保存（24 个月）。收货后建议分装。避免冷冻/解冻循环。
图片
Alpha smooth muscle Actin Mouse mAb (Ab088443) - Western Blot All lanes: Alpha smooth muscle Actin Mouse mAb (Ab088443) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution
Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 5.6 s
Alpha smooth muscle Actin Mouse mAb (Ab088443) - Western Blot All lanes: Alpha smooth muscle Actin Mouse mAb (Ab088443) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 26.7 s
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Human Ovarian cancer Tissue Primary Ab:10ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Human Rectum Tissue Primary Ab:10ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Rat Skeletal muscle Tissue Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Rat Lung Tissue Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Mouse Pancreas Tissue Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Mouse Spleen Tissue Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
ZJ24F0911292
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