Rabbit anti Human APPL Antibody, Recombinant, could be used for WB, IHC and so on. Application:
WB: 1/500-1/1000 IHC: 1/50-1/100
IF/ICC: 1/200
Protein Function Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction.
Recombinant APPL Antibody (Ab089390) - Western Blot All lanes: Recombinant APPL Antibody (Ab089390) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 80 kDa Observed band size: 87 kDa Exposure time: 60.0 s
Recombinant APPL Antibody (Ab089390) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant APPL Antibody (Ab089390). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant APPL Antibody (Ab089390) - IF/ICC IF analysis of Recombinant APPL Antibody (Ab089390) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant APPL Antibody (Ab089390) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).