Rabbit anti Human Aprataxin Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. Also able to hydrolyze adenosine 5'-monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity.
Recombinant Aprataxin Antibody (Ab089394) - Western Blot All lanes: Recombinant Aprataxin Antibody (Ab089394) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 41 kDa Observed band size: 42 kDa Exposure time: 24.8 s
Recombinant Aprataxin Antibody (Ab089394) - IF/ICC Immunocytochemistry analysis of Aprataxin (green) in U87-MG using Recombinant Aprataxin Antibody (Ab089394), and cells were counterstained with DAPI (blue).