actin-dependent regulator of chromatin subfamily F member 1 antibody | ARI1A_HUMAN antibody | ARID domain containing protein 1A antibody | ARID domain-containing protein 1A antibody | ARID1A antibody | AT rich interactive domain 1A (SWI like) antibody | A
规格或纯度
重组, ExactAb™, 已验证, 见COA
生化机理
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Binds DNA non-specifically. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
宿主种属
兔(Rabbit)
特异性
ARID1A
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human ARID1A (AA 1200-1400).
阳性对照
WB: HEK293, Jurkat and HeLa cell lysates. IHC: Human colon adenocarcinoma tissue. IF/ICC: SH-SY5Y cells.
Recombinant ARID1A Antibody (Ab089579) - Western Blot All lanes: Recombinant ARID1A Antibody (Ab089579) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 242 kDa Observed band size: 132, 150, 270 kDa ARID1A has many mutations which typically generate truncated proteins that are highly prone to degradation. (PMID: 21614196, PMID: 29486633, PMID: 34429326). Exposure time: 17.3 s
Recombinant ARID1A Antibody (Ab089579) - Western Blot All lanes use Recombinant ARID1A Antibody (Ab089579) at 1/1000 dilution for 1 hour at room temperature.
Recombinant ARID1A Antibody (Ab089579) - IHC Immunohistochemical analysis of paraffin-embedded human adenocarcinoma of colon, using Recombinant ARID1A Antibody (Ab089579).
Recombinant ARID1A Antibody (Ab089579) - IF/ICC Immunofluorescent analysis of SH-SY5Y cells, using Recombinant ARID1A Antibody (Ab089579).
ZJ24F1012750
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