ExactAb™,已验证,无叠氮钠,无载体 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human ARPC4 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 0.01-2µg/mL
IHC: 5-20µg/mL
IF/ICC: 2.3µg/mL
Protein Function
Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.
ARPC4 Antibody (Ab089682) - Western Blot
All lanes: ARPC4 Antibody (Ab089682) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 19 kDa
Exposure time: 5.0 s
ARPC4 Antibody (Ab089682) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 20 µg/mL ARPC4 Antibody (Ab089682)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
ARPC4 Antibody (Ab089682) - IHC
DAB staining on IHC
Sample: Human Liver Tissue
Primary Ab: 20 µg/mL ARPC4 Antibody (Ab089682)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
ARPC4 Antibody (Ab089682) - IF/ICC
IF analysis of ARPC4 Antibody (Ab089682) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with ARPC4 Antibody (Ab089682) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
5-20 µg/mL
0.01-2 µg/mL
2.3µg/mL
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0708816 |
分析证书 | Ab089682 |
| ZJ24F0708817 |
分析证书 | Ab089682 |
| ZJ24F0708818 |
分析证书 | Ab089682 |
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