ARRDC1 Antibody (Ab089692) - Western Blot All lanes: ARRDC1 Antibody (Ab089692) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution Predicted band size: 45 kDa Observed band size: 48 kDa Exposure time: 86.8 s
ARRDC1 Antibody (Ab089692) - IF/ICC Immunofluorescent analysis of ARRDC1 staining in MDA-MB-231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ARRDC1 Antibody (Ab089692) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).