Rabbit anti Human ARSA/ASA Antibody, Polyclonal, could be used for WB, IHC and so on. Application:
WB: 0.01-2µg/mL
IHC: 5-20µg/mL IF/ICC: 2.5µg/mL Protein Function Hydrolyzes cerebroside sulfate.
规格
产品名称
ARSA/ASA Antibody
别名
芳基硫酸酯酶A(ARSA)抗体 | ARSA/ASA 抗体
英文别名
ARSA_HUMAN antibody | arylsulfatase A antibody | Arylsulfatase A component C antibody | As 2 antibody | AS A antibody | As2 antibody | ASA antibody | AW212749 antibody | C230037L18Rik antibody | Cerebroside-sulfatase antibody | metachromatic leucodystroph
规格或纯度
无载体, ExactAb™, 无叠氮钠, 已验证, 见COA
宿主种属
兔(Rabbit)
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Recombinant ARSA expressed in E.coli (AA 19-293)
阳性对照
WB: Mouse kidney, Mouse liver and Rat kidney lysates. IHC: Human esophagus, prostate cancer, glioma, liver, stomach tissues. IF/ICC: HeLa cells.
偶联
Unconjugated
克隆类型
多克隆抗体
Format
Whole IgG
亚型
Rabbit IgG
纯化方法
Antigen affinity purified + Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4
防腐剂
0.05% Proclin 300
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
图片
ARSA/ASA Antibody (Ab089702) - Western Blot All lanes: ARSA/ASA Antibody (Ab089702) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 54 kDa Observed band size: 53 kDa Exposure time: 54.4 s
ARSA/ASA Antibody (Ab089702) - IHC DAB staining on IHC Samples: Human Esophagus Tissue Primary Ab: 20 µg/mL ARSA/ASA Antibody (Ab089702) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
ARSA/ASA Antibody (Ab089702) - IHC DAB staining on IHC Samples: Human Prostate cancer Tissue Primary Ab: 20 µg/mL ARSA/ASA Antibody (Ab089702) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
ARSA/ASA Antibody (Ab089702) - IHC DAB staining on IHC Samples: Human Glioma Tissue Primary Ab: 20 µg/mL ARSA/ASA Antibody (Ab089702) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
ARSA/ASA Antibody (Ab089702) - IHC DAB staining on IHC Samples: Human Liver Tissue Primary Ab: 20 µg/mL ARSA/ASA Antibody (Ab089702) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
ARSA/ASA Antibody (Ab089702) - IHC DAB staining on IHC Samples: Human Stomach Tissue Primary Ab: 20 µg/mL ARSA/ASA Antibody (Ab089702) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
ARSA/ASA Antibody (Ab089702) - IHC DAB staining on IHC Sample: Human Liver Tissue Primary Ab: 20 µg/mL ARSA/ASA Antibody (Ab089702) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
ARSA/ASA Antibody (Ab089702) - IHC DAB staining on IHC Sample: Human Stomach Tissue Primary Ab: 20 µg/mL ARSA/ASA Antibody (Ab089702) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
ARSA/ASA Antibody (Ab089702) - IF/ICC IF analysis of ARSA/ASA Antibody (Ab089702) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with ARSA/ASA Antibody (Ab089702) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).