Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.
宿主种属
兔(Rabbit)
特异性
ATF6
种属反应性
人(Human)
免疫原
Recombinant protein of human ATF6 (AA 1-333)
阳性对照
WB: A549, HEK293, U2OS and HeLa cell lysates. IHC: Human lung cancer tissue. IF/ICC: HeLa cells.
Recombinant ATF6 Antibody (Ab089961) - Western Blot All lanes: Recombinant ATF6 Antibody (Ab089961) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 75 kDa Observed band size: 98 kDa Exposure time: 60.0 s
Recombinant ATF6 Antibody (Ab089961) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant ATF6 Antibody (Ab089961). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant ATF6 Antibody (Ab089961) - IF/ICC IF analysis of Recombinant ATF6 Antibody (Ab089961) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant ATF6 Antibody (Ab089961) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).