ExactAb™,已验证,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human ATG3 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function：
E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
Recombinant ATG3 Antibody (Ab089994) - Western Blot
All lanes: Recombinant ATG3 Antibody (Ab089994) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 49.5 s
Recombinant ATG3 Antibody (Ab089994) - IF/ICC
Immunocytochemistry analysis of ATG3 (green) in HeLa using Recombinant ATG3 Antibody (Ab089994) and DAPI (blue).
Recombinant ATG3 Antibody (Ab089994) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant ATG3 Antibody (Ab089994). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
1/500-1/1000
1/50 - 1/100
1/50-1/200
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0809165 |
分析证书 | Ab089994 |
| ZJ24F0809166 |
分析证书 | Ab089994 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Recombinant 级别指南 → 查看 Validated 级别指南 → 查看 ExactAb™ 级别指南 →
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