Rabbit anti Human ATP5H Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/500-1/2000 IHC: 1/100-1/300 Protein Function: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
规格
产品名称
ATP5H Antibody
别名
ATP5PD抗体 | ATP5H抗体 | ATP5H抗体
英文别名
ATP synthase D chain mitochondrial antibody | ATP synthase H+ transporting mitochondrial F1F0 subunitantibody | ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody | ATP synthase subunit d antibody | ATP synthase subunit d, mitochondrial an
规格或纯度
ExactAb™, Validated, 1.0 mg/mL
宿主种属
兔(Rabbit)
特异性
ATP5H
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human ATP5H (AA 111-160).
阳性对照
WB: Hep G2, Jurkat and HeLa cell lysates. IHC: Human Squamous cell carcinoma of lung tissue.
ATP5H Antibody (Ab090134) - Western Blot All lanes: ATP5H Antibody (Ab090134) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 26 kDa Observed band size: 22 kDa Exposure time: 14.0 s
ATP5H Antibody (Ab090134) - IHC Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1. ATP5H Antibody (Ab090134) was diluted at 1/200 (4 ℃, overnight). 2. Tris-EDTA, pH 9.0 was used for antigen retrieval. 3. Secondary antibody was diluted at 1/200 (room temperature, 45 min).