Rabbit anti Human ATP6V0D1 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis (By similarity). May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium.
规格
产品名称
Recombinant ATP6V0D1/P39 Antibody
别名
兔单克隆抗体 to ATP6V0D1/P39 | 重组ATP6V0D1/P39抗体
英文别名
32 kDa accessory protein antibody | ATP6D antibody | ATP6DV antibody | ATP6V0D1 antibody | ATPase H+ transporting lysosomal (vacuolar proton pump) member D antibody | ATPase H+ transporting lysosomal 38kD V0 subunit d antibody | ATPase H+ transporting lys
规格或纯度
ExactAb™, Validated, 重组, 0.7 mg/mL
宿主种属
兔(Rabbit)
特异性
ATP6V0D1
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Recombinant protein of human ATP6V0D1 (AA 1-351)
阳性对照
WB: K562, HeLa and MCF7 cell lysates. IHC: Human colon cancer tissue. ICC/IF: HeLa cells.
Recombinant ATP6V0D1/P39 Antibody (Ab090154) - Western Blot All lanes: Recombinant ATP6V0D1/P39 Antibody (Ab090154) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 40 kDa Observed band size: 37 kDa Exposure time: 9.1 s
Recombinant ATP6V0D1/P39 Antibody (Ab090154) - IF/ICC Immunocytochemistry analysis of ATP6V0D1 (green) in HeLa using Recombinant ATP6V0D1/P39 Antibody (Ab090154), and cells were counterstained with DAPI (blue).
Recombinant ATP6V0D1/P39 Antibody (Ab090154) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant ATP6V0D1/P39 Antibody (Ab090154). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.