Rabbit anti Human ATP6V1D Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/500-1/4000 IHC: 1/50-1/100 Protein Function Relevance': ""ATP6V1D is a subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.
规格
产品名称
ATP6V1D Antibody
别名
兔抗ATP6V1D多克隆抗体 | ATP6V1D 抗体
英文别名
ATP6M antibody | ATP6V1D antibody | V-ATPase 28 kDa accessory proteinantibody | V-ATPase subunit D antibody | V-type proton ATPase subunit D antibody | Vacuolar ATP synthase subunit D antibody | Vacuolar proton pump subunit D antibody | VATD antibody | VM
规格或纯度
ExactAb™, Validated, Carrier Free, High performance, 0.9 mg/mL
宿主种属
兔(Rabbit)
特异性
ATP6V1D
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Fusion protein of human ATP6V1D (AA 169-238).
阳性对照
WB: Mouse brain tissue lysate, NIH/3T3, C6, HeLa and HEK293 cell lysates. IHC: Human colorectal cancer tissue. IF/ICC: HeLa cells.
ATP6V1D Antibody (Ab090177) - Western Blot All lanes: ATP6V1D Antibody (Ab090177) at 1/4000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa Observed band size: 41 kDa Exposure time: 113.8 s
ATP6V1D Antibody (Ab090177) - Western Blot All lanes: ATP6V1D Antibody (Ab090177) at 1/4000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa Observed band size: 41 kDa Exposure time: 17.7 s
ATP6V1D Antibody (Ab090177) - IHC Immunohistochemistry analysis of paraffin-embedded Human colorectal cancer using ATP6V1D Antibody (Ab090177). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
ATP6V1D Antibody (Ab090177) - IF/ICC
IF analysis of ATP6V1D Antibody (Ab090177) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with ATP6V1D Antibody (Ab090177) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).