ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody | ATP synthase subunit beta mitochondrial antibody | ATP synthase subunit beta, mitochondrial antibody | atp5b antibody | ATPB antibody | ATPB_HUMAN antibody | ATPMB antibody
规格或纯度
重组, ExactAb™, 已验证, 高性能, 见COA
生化机理
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
宿主种属
兔(Rabbit)
特异性
ATP5F1B
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse),猴(Monkey)
免疫原
A synthetic peptide derived from human ATPB (AA 151-164)
阳性对照
WB: Jurkat, HeLa, Raji, Hep G2, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human tonsil tissue. ICC/IF: HeLa cells.
Recombinant ATPB Antibody (Ab090212) - Western Blot All lanes: Recombinant ATPB Antibody (Ab090212) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 57 kDa Observed band size: 52 kDa Exposure time: 1.9 s
Recombinant ATPB Antibody (Ab090212) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant ATPB Antibody (Ab090212). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant ATPB Antibody (Ab090212) - IF/ICC Immunocytochemistry analysis of ATPB (green) in HeLa cells using Recombinant ATPB Antibody (Ab090212) and DAPI (blue).
ZJ24F0910117
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