Recombinant AVEN Antibody (Ab090302) - Western Blot All lanes: Recombinant AVEN Antibody (Ab090302) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 39 kDa Observed band size: 50 kDa Exposure time: 8.8 s
Recombinant AVEN Antibody (Ab090302) - IHC Immunohistochemistry analysis of paraffin-embedded mouse testis using Recombinant AVEN Antibody (Ab090302). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant AVEN Antibody (Ab090302) - IF/ICC IF analysis of Recombinant AVEN Antibody (Ab090302) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant AVEN Antibody (Ab090302) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).