Rabbit anti Human Bcl10 Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/200
Protein Function
Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.
Recombinant Bcl10 Antibody (Ab090757) - Western Blot All lanes: Recombinant Bcl10 Antibody (Ab090757) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 26 kDa Observed band size: 30 kDa Exposure time: 85.0 s
Recombinant Bcl10 Antibody (Ab090757) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant Bcl10 Antibody (Ab090757). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant Bcl10 Antibody (Ab090757) - IF/ICC
IF analysis of Recombinant Bcl10 Antibody (Ab090757) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Bcl10 Antibody (Ab090757) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).