Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
宿主种属
兔(Rabbit)
特异性
Bcl-2
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human Bcl-2 (AA 48-81)
阳性对照
WB: HeLa, A375, Jurkat, MCF7, THP-1, U937, NIH/3T3 and C6 cell lysates. IHC: Rat liver, stomach, Human non-Hodgkin's lymphoma, squamous cell carcinoma, spleen, Mouse skin tissues. IF/ICC: Jurkat cells.
Recombinant Bcl-2 Antibody (Ab090774) - Western Blot All lanes: Recombinant Bcl-2 Antibody (Ab090774) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 26 kDa Observed band size: 17, 26, 59 kDa Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (PubMed:25609812, PubMed:8183370). Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. Exposure time: 9.3 s
Recombinant Bcl-2 Antibody (Ab090774) - IHC Immunohistochemical analysis of paraffin-embedded Rat liver, using Recombinant Bcl-2 Antibody (Ab090774) at 1/100 dilution.
Recombinant Bcl-2 Antibody (Ab090774) - IHC Immunohistochemical analysis of paraffin-embedded Rat stomach, using Recombinant Bcl-2 Antibody (Ab090774) at 1/100 dilution.
Recombinant Bcl-2 Antibody (Ab090774) - IHC Immunohistochemical analysis of paraffin-embedded Human non-Hodgkin's lymphoma, using Recombinant Bcl-2 Antibody (Ab090774) at 1/100 dilution.
Recombinant Bcl-2 Antibody (Ab090774) - IHC Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma , using Recombinant Bcl-2 Antibody (Ab090774) at 1/100 dilution.
Recombinant Bcl-2 Antibody (Ab090774) - IHC Immunohistochemical analysis of paraffin-embedded Mouse skin, using Recombinant Bcl-2 Antibody (Ab090774) at 1/100 dilution.
Recombinant Bcl-2 Antibody (Ab090774) - IHC Immunohistochemical analysis of paraffin-embedded human spleen, using Recombinant Bcl-2 Antibody (Ab090774).
Recombinant Bcl-2 Antibody (Ab090774) - IF/ICC Immunofluorescent analysis of Jurkat cells, using Recombinant Bcl-2 Antibody (Ab090774).
ZJ25F0521417
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