Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Post-translational: Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear. Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
宿主种属
兔(Rabbit)
特异性
beta Tubulin
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human beta Tubulin (AA 300-360).
阳性对照
WB: MCF7, Jurkat, HeLa, A549, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Human glioma, uterus cancer, Rat brain, Mouse kidney tissue. IF/ICC: NIH/3T3 cells.
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant beta Tubulin Antibody (Ab091196) - Western Blot All lanes: Recombinant beta Tubulin Antibody (Ab091196) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 100.0 s
Recombinant beta Tubulin Antibody (Ab091196) - IHC Immunohistochemical analysis of paraffin-embedded (1) Human glioma; (2) Rat brain; (3) Mouse kidney; (4) Human uterus cancer, using Recombinant beta Tubulin Antibody (Ab091196).
Recombinant beta Tubulin Antibody (Ab091196) - IF/ICC Immunofluorescent analysis of NIH/3T3 cells, using Recombinant beta Tubulin Antibody (Ab091196).
ZJ25F0521219
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