Rabbit anti Human Bmi1 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function: Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
规格
产品名称
Recombinant Bmi1 Antibody
别名
Bmi1抗体 | 组蛋白相关Bmi1抗体 | 多梳家族环指蛋白4抗体 | 重组Bmi1抗体
英文别名
Polycomb group RING finger protein 4 | RING finger protein 51 | B lymphoma Mo MLV insertion region (mouse) antibody | B lymphoma Mo MLV insertion region 1 homolog antibody | Bmi 1 antibody | BMI1 antibody | BMI1 polycomb ring finger oncogene antibody | BM
规格或纯度
ExactAb™, Validated, 重组, 0.7 mg/mL
宿主种属
兔(Rabbit)
特异性
Bmi1
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Recombinant protein of human Bmi1
阳性对照
WB: K562, HeLa, U-2 OS, MCF7, NIH/3T3 and C6 cell lysates. ICC/IF: HeLa cells.
Recombinant Bmi1 Antibody (Ab091398) - Western Blot All lanes: Recombinant Bmi1 Antibody (Ab091398) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 37 kDa Observed band size: 40 kDa Exposure time: 35.5 s
Recombinant Bmi1 Antibody (Ab091398) - IF/ICC Immunocytochemistry analysis of BMI1 (green) in HeLa using Recombinant Bmi1 Antibody (Ab091398) and DAPI (blue).