Rabbit anti Human Caveolin-1 Antibody, Polyclonal antibody, could be used for WB, IHC and so on. Application WB: 0.03-2 µg/mL
IHC: 5-20 µg/mL
IF/ICC: 5 µg/mL Protein Function May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
WB: Mouse lung, A431 and A549 lysates and Mouse lung. IHC: Human Thyroid cancer tissue, Human Breast cancer tissue, Mouse Colon tissue, Mouse Cardiac Muscle tissue, Porcine Cerebrum tissue, Porcine Uterus tissue, Human Placenta tissue, Porcine Colon tissu
偶联
Unconjugated
克隆类型
多克隆抗体
Format
Whole IgG
亚型
Rabbit IgG
纯化方法
Antigen affinity purified and protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4
防腐剂
0.05% Proclin 300
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
图片
Caveolin-1 Antibody (Ab093242) - Western Blot All lanes: Caveolin-1 Antibody (Ab093242) at 1/5000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 20 kDa Observed band size: 19&21 kDa Exposure time: 1.8 s
Caveolin-1 Antibody (Ab093242) - Western Blot All lanes: Caveolin-1 Antibody (Ab093242) at 1/5000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 20 kDa Observed band size: 22 kDa Exposure time: 1.8 s
Caveolin-1 Antibody (Ab093242) - IHC DAB staining on IHC Samples: Human Thyroid cancer Tissue Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Caveolin-1 Antibody (Ab093242) - IHC DAB staining on IHC Samples: Human Breast cancer Tissue Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Caveolin-1 Antibody (Ab093242) - IHC DAB staining on IHC Sample: Mouse Colon Tissue Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Caveolin-1 Antibody (Ab093242) - IHC DAB staining on IHC Sample: Mouse Cardiac Muscle Tissue Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Caveolin-1 Antibody (Ab093242) - IHC DAB staining on IHC Sample: Porcine Cerebrum Tissue Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Caveolin-1 Antibody (Ab093242) - IHC DAB staining on IHC Sample: Porcine Uterus Tissue Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Caveolin-1 Antibody (Ab093242) - IHC DAB staining on IHC Sample: Human Placenta Tissue Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Caveolin-1 Antibody (Ab093242) - IHC DAB staining on IHC Sample: Porcine Colon Tissue Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Caveolin-1 Antibody (Ab093242) - IHC DAB staining on IHC Sample: Porcine Uterus Tissue Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Caveolin-1 Antibody (Ab093242) - IHC DAB staining on IHC Sample: Porcine Small intestine Tissue Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Caveolin-1 Antibody (Ab093242) - IHC DAB staining on IHC Sample: Porcine Cardiac Muscle Tissue Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Caveolin-1 Antibody (Ab093242) - IHC DAB staining on IHC Sample: Porcine Lymph node Tissue Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Caveolin-1 Antibody (Ab093242) - IHC DAB staining on IHC Sample: Porcine Liver Tissue Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Caveolin-1 Antibody (Ab093242) - IHC DAB staining on IHC Sample: Porcine Lung Tissue Primary Ab: 20µg/mL Caveolin-1 Antibody (Ab093242) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Caveolin-1 Antibody (Ab093242) - IF/ICC
IF analysis of Caveolin-1 Antibody (Ab093242) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Caveolin-1 Antibody (Ab093242) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).