NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione.
宿主种属
小鼠(Mouse)
特异性
CBR1
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Recombinant human CBR1 protein fragments expressed in E.coli
阳性对照
WB: HeLa, A431, HEK293, A549, MCF7, NIH/3T3 and C6 cell lysates. IF/ICC: HeLa cells.
CBR1 Mouse mAb (Ab093315) - Western Blot All lanes: CBR1 Mouse mAb (Ab093315) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 30 kDa Observed band size: 29 kDa Exposure time: 13.1 s
CBR1 Mouse mAb (Ab093315) - IF/ICC Immunofluorescent analysis of CBR1 Mouse mAb (Ab093315) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5 - 10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
ZJ24F0809735
危险品化学品经营许可证（带存储）